SMO Antibody - #DF5152

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产品: SMO 抗体
货号: DF5152
描述: Rabbit polyclonal antibody to SMO
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Chicken
分子量: 86 KD; 86kD(Calculated).
蛋白号: Q99835
RRID: AB_2837342

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
SMO Antibody detects endogenous levels of total SMO.
RRID:
AB_2837342
引用格式: Affinity Biosciences Cat# DF5152, RRID:AB_2837342.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Frizzled family member 11; FZD11; Gx; Gx protein; Protein Gx; SMO; SMO_HUMAN; SMOH; Smoothened frizzled family receptor; Smoothened homolog (Drosophila); Smoothened homolog; Smoothened homolog precursor (SMO) (Gx protein); Smoothened seven transmembrane spanning receptor;

抗原和靶标

免疫原:
Uniprot:

Q99835(SMO_HUMAN) >>Visit HPA database.

基因/基因ID:
SMO(6608)
序列:
MAAARPARGPELPLLGLLLLLLLGDPGRGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTSNETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKQPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Chicken
100
Rabbit
100
Xenopus
63
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99835 作为底物

Site PTM Type Enzyme
S32 Phosphorylation
S43 Phosphorylation
S47 Phosphorylation
S588 Phosphorylation
S590 Phosphorylation
T593 Phosphorylation
S595 Phosphorylation
S611 Phosphorylation
S615 Phosphorylation
S616 Phosphorylation
K674 Acetylation
S771 Phosphorylation
S785 Phosphorylation

研究背景

功能:

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

细胞定位:

Membrane>Multi-pass membrane protein. Cell projection>Cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with ARRB2 (By similarity). Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO. Interacts with DLG5 and SDCBP (By similarity). Interacts with GAS8/DRC4.

蛋白家族:

Belongs to the G-protein coupled receptor Fz/Smo family.

研究领域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death & Disease (PubMed: 31366904) [IF=9.0]

Application: WB    Species: mouse    Sample: BGC823MCAs and SGC7901MCAs

Fig. S5 |a Western blottingting showing the most pronounced effect of Smo silencing in the shSmo #2 group in BGC823MCAs and SGC7901MCAs.
2). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology (PubMed: 36278239) [IF=5.6]
3). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH (PubMed: 31403228) [IF=3.8]

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.
4). Disruption of Gprasp2 down-regulates Hedgehog signaling and leads to apoptosis in auditory cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 34418635) [IF=3.1]

Application: WB    Species: Mice    Sample: HEI-OC1 cells

Fig. 4. Gprasp2-KO induced apoptosis in HEI-OC1 cells by inhibiting Hh signaling pathway. (A) Flow cytometry assay of Gprasp2-WT/KO HEI-OC1 cells with no PUR-treatments (WT, KO) or with PUR-treatments (WT þ PUR, KO þ PUR). (B) Statistics of apoptotic cells in WT/KO/WT þ PUR/KO þ PUR groups (n ¼ 3, *p < 0.05). (C) Western blot assay of the key molecules of apoptosis (Bcl2, Bax, Casepase3, cleaved-Casepase3) and Hh signaling pathway (Smo, Gli1, Gli2) in WT/KO/WT þ PUR/KO þ PUR groups. (D) Statistics of the key molecules of apoptosis (Bcl2, Bax, Casepase3, cleaved-Casepase3) and Hh signaling pathway (Smo, Gli1, Gli2) in WT/KO/WT þ PUR/KO þ PUR groups (n ¼ 3; *p < 0.05).
5). Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells. Oncology Letters (PubMed: 31452785) [IF=2.9]
6). Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer. Translational cancer research (PubMed: 37969369) [IF=0.9]

Application: WB    Species: Mouse    Sample: liver cancer cell

Figure 2 Ubenimex blocked Hh signaling by targeting CD13 in liver cancer cell lines. (A-C) Western blot analysis of the protein expression levels of CD13, PTCH1, GLI1, SMO, and SHH in Huh7, HepG2, and PLC/PRF/5 cells treated with 0.05 or 0.1 mg/mL ubenimex. Densitometry analysis was used to normalize the expression levels of the proteins to those of β-actin; mean ± SD is indicative of three independent experiments (*, P

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