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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF5046, RRID:AB_2837405.
展开/折叠
CSNBAD1; MGC138309; MGC138311; OPN 2; OPN2; opsd; OPSD_HUMAN; opsin 2; Opsin 2 rod pigment; Opsin-2; Opsin2; Retinitis Pigmentosa 4; Retinitis pigmentosa 4 autosomal dominant; RHO; Rhodopsin; RP 4; RP4;
抗原和靶标
A synthesized peptide derived from human RHO, corresponding to a region within C-terminal amino acids.
- P08100 OPSD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08100 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N15 | N-Glycosylation | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Q15835 (GRK1) , P17252 (PRKCA) | Uniprot |
| T336 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S338 | Phosphorylation | Q15835 (GRK1) | Uniprot |
| T340 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Q15835 (GRK1) | Uniprot |
研究背景
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region (By similarity). After activation by light, phosphorylated by GRK1 (in vitro).
Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.
Membrane>Multi-pass membrane protein. Cell projection>Cilium>Photoreceptor outer segment.
Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.
Rod shaped photoreceptor cells which mediate vision in dim light.
Homodimer (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity). Interacts with GRK1. Interacts (phosphorylated form) with SAG. Interacts with GNAT1. Interacts with GNAT3. SAG and G-proteins compete for a common binding site. Interacts with PRCD; the interaction promotes PRCD stability (By similarity).
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
研究领域
· Organismal Systems > Sensory system > Phototransduction.
文献引用
Application: WB Species: Human Sample: mesenchymal stem cells
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