产品: TAS2R14 抗体
货号: DF5159
描述: Rabbit polyclonal antibody to TAS2R14
应用: WB IF/ICC
反应: Human
分子量: 38 KD; 36kD(Calculated).
蛋白号: Q9NYV8
RRID: AB_2837508

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
TAS2R14 Antibody detects endogenous levels of total TAS2R14.
RRID:
AB_2837508
引用格式: Affinity Biosciences Cat# DF5159, RRID:AB_2837508.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

T2R14; T2R14_HUMAN; TAS2R14; Taste receptor family B member 1; Taste receptor type 2 member 14; Taste receptor, family B, member 1; Taste receptor, type 2, member 14; TRB1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9NYV8 T2R14_HUMAN:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis (PubMed:16720576).

序列:
MGGVIKSIFTFVLIVEFIIGNLGNSFIALVNCIDWVKGRKISSVDRILTALAISRISLVWLIFGSWCVSVFFPALFATEKMFRMLTNIWTVINHFSVWLATGLGTFYFLKIANFSNSIFLYLKWRVKKVVLVLLLVTSVFLFLNIALINIHINASINGYRRNKTCSSDSSNFTRFSSLIVLTSTVFIFIPFTLSLAMFLLLIFSMWKHRKKMQHTVKISGDASTKAHRGVKSVITFFLLYAIFSLSFFISVWTSERLEENLIILSQVMGMAYPSCHSCVLILGNKKLRQASLSVLLWLRYMFKDGEPSGHKEFRESS

翻译修饰 - Q9NYV8 作为底物

Site PTM Type Enzyme
T49 Phosphorylation
Y121 Phosphorylation
N153 N-Glycosylation
T164 Phosphorylation
S170 Phosphorylation
T173 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
Y300 Phosphorylation
S308 Phosphorylation

研究背景

功能:

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis.

蛋白家族:

Belongs to the G-protein coupled receptor T2R family.

研究领域

· Organismal Systems > Sensory system > Taste transduction.

文献引用

1). Biomimetic in vitro respiratory system using smooth muscle cells on ECIS chips for anti-asthma TCMs screening. Analytica Chimica Acta, 2021 (PubMed: 33926702) [IF=6.2]

Application: IF/ICC    Species: Mouse    Sample: ASM cells

Fig. 2. Identification of TAS2Rs in mouse ASM cells. (A) RT-qPCR of mTAS2Rs expression in mouse ASM cells, with b-actin as internal control. Results were normalized to expression of the Adora1. Data are collected from three independent experiments. (B) Immunostaining with DAPI (blue), anti-TAS2R10, and anti-TAS2R14 receptor antibody (red), scale bar ¼ 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

2). Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2. Food & Function, 2021 (PubMed: 34881772) [IF=6.1]

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.