产品: Cytokeratin 13 抗体
货号: AF5102
描述: Rabbit polyclonal antibody to Cytokeratin 13
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Dog
分子量: 50 kDa; 50kD(Calculated).
蛋白号: P13646
RRID: AB_2837588

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(82%), Dog(91%)
克隆:
Polyclonal
特异性:
Cytokeratin 13 Antibody detects endogenous levels of total Cytokeratin 13.
RRID:
AB_2837588
引用格式: Affinity Biosciences Cat# AF5102, RRID:AB_2837588.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

47 kDa cytokeratin; CK-13; CK13; Cytokeratin 13; Cytokeratin-13; K13; K1C13_HUMAN; Ka13; Keratin 13; Keratin; keratin type I cytoskeletal 13; Keratin-13; Krt-1.13; Krt1-13; KRT13; MGC161462; MGC3781; type I cytoskeletal 13; Type I keratin Ka13; WSN2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P13646 K1C13_HUMAN:

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

描述:
Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains.
序列:
MSLRLQSSSASYGGGFGGGSCQLGGGRGVSTCSTRFVSGGSAGGYGGGVSCGFGGGAGSGFGGGYGGGLGGGYGGGLGGGFGGGFAGGFVDFGACDGGLLTGNEKITMQNLNDRLASYLEKVRALEEANADLEVKIRDWHLKQSPASPERDYSPYYKTIEELRDKILTATIENNRVILEIDNARLAADDFRLKYENELALRQSVEADINGLRRVLDELTLSKTDLEMQIESLNEELAYMKKNHEEEMKEFSNQVVGQVNVEMDATPGIDLTRVLAEMREQYEAMAERNRRDAEEWFHTKSAELNKEVSTNTAMIQTSKTEITELRRTLQGLEIELQSQLSMKAGLENTVAETECRYALQLQQIQGLISSIEAQLSELRSEMECQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMIGFPSSAGSVSPRSTSVTTTSSASVTTTSNASGRRTSDVRRP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Dog
91
Horse
82
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13646 作为底物

Site PTM Type Enzyme
S7 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
Y12 Phosphorylation
R27 Methylation
R35 Methylation
Y118 Phosphorylation
S144 Phosphorylation
S147 Phosphorylation
Y152 Phosphorylation
S153 Phosphorylation
Y155 Phosphorylation
Y156 Phosphorylation
T168 Phosphorylation
T170 Phosphorylation
Y194 Phosphorylation
Y238 Phosphorylation
Y281 Phosphorylation
T311 Phosphorylation
T316 Phosphorylation
T327 Phosphorylation
S337 Phosphorylation
S340 Phosphorylation
T404 Phosphorylation
Y405 Phosphorylation
S421 Phosphorylation
S422 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
T434 Phosphorylation
T452 Phosphorylation
S453 Phosphorylation

研究背景

翻译修饰:

O-glycosylated; glycans consist of single N-acetylglucosamine residues.

组织特异性:

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

亚基结构:

Heterotetramer of two type I and two type II keratins. keratin-13 is generally associated with keratin-4.

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Cytokeratin 13 promotes radiotherapy sensitivity of nasopharyngeal carcinoma by downregulating the MEK/ERK pathway. IUBMB LIFE, 2022 (PubMed: 35426961) [IF=4.6]

Application: WB    Species: Human    Sample: NPC cells

FIGURE 1CK13 promoted the sensitivity of NPC HNE-3 cells to radiotherapy. (a, b) qRT-PCR and Western blotting detected the mRNA expression and protein levels of CK13 in a variety of NPC cells, respectively. Asterisks represent statistically significant differences (*p 

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