Insulin Antibody - #AF5109

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产品: Insulin 抗体
货号: AF5109
描述: Rabbit polyclonal antibody to Insulin
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 11 kDa; 12kD(Calculated).
蛋白号: P01308
RRID: AB_2837595

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(80%), Sheep(80%), Rabbit(80%), Dog(80%)
克隆:
Polyclonal
特异性:
Insulin Antibody detects endogenous levels of total Insulin.
RRID:
AB_2837595
引用格式: Affinity Biosciences Cat# AF5109, RRID:AB_2837595.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IDDM; IDDM1; IDDM2; ILPR; ins; INS_HUMAN; Insulin A chain; Insulin B chain; IRDN; MODY10; Preproinsulin; Proinsulin; Proinsulin precursor;

抗原和靶标

免疫原:
Uniprot:

P01308(INS_HUMAN) >>Visit HPA database.

基因/基因ID:
INS(3630)
描述:
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
序列:
MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Horse
70
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01308 作为底物

Site PTM Type Enzyme
S98 Phosphorylation
Y108 Phosphorylation

研究背景

功能:

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

蛋白家族:

Belongs to the insulin family.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). Celastrol targets the ChREBP-TXNIP axis to ameliorates type 2 diabetes mellitus. Phytomedicine, 2023 (PubMed: 36603341) [IF=7.9]
2). Palmatine ameliorates high fat diet induced impaired glucose tolerance. Biological research, 2020 (PubMed: 32928312) [IF=6.7]

Application: IHC    Species: Rat    Sample:

Fig. 2 Effect of PAL on the insulin resistance and β-cell mass in high fat diet induced rats. aThe blood insulin levels of three groups. b The HOMA-IR in three groups. c The β cells mass in each group determined by immunohistochemical staining. Scale bar: 50 μm. d Quantification of the β cell mass. e Cell apoptosis in β cells determined by TUNEL/insulin immunofluorescent staining. Scale bar: 50 μm. f Quantification of TUNEL-postive β cells. Values are expressed as mean ± SD. n = 6. Compared with control group: #P 

Application: IF/ICC    Species: Rat    Sample:

Fig. 2 Effect of PAL on the insulin resistance and β-cell mass in high fat diet induced rats. aThe blood insulin levels of three groups. b The HOMA-IR in three groups. c The β cells mass in each group determined by immunohistochemical staining. Scale bar: 50 μm. d Quantification of the β cell mass. e Cell apoptosis in β cells determined by TUNEL/insulin immunofluorescent staining. Scale bar: 50 μm. f Quantification of TUNEL-postive β cells. Values are expressed as mean ± SD. n = 6. Compared with control group: #P 
3). Metformin inhibits MAPK signaling and rescues pancreatic aquaporin 7 expression to induce insulin secretion in type 2 diabetes mellitus. Journal of Biological Chemistry, 2021 (PubMed: 34303707) [IF=4.8]

Application: IF/ICC    Species: Rat    Sample: INS-1 cells

Figure 1 AQP7 expression in pancreatic islets. Double immunofluorescent staining for AQP7 in red and insulin (a marker of pancreatic β-cell) in green showed AQP7 was colocalized with insulin in yellow. Arrows show AQP7 detectable in pancreatic β-cell marker. Nuclei are labeled by DAPI in blue.A, bar graph shows a quantification of insulin fluorescence. B, bar graph shows a quantification of AQP7 fluorescence. Control group (Ctrl), untreated diabetic group (T2DM), 0.9% normal saline-treated diabetic group (T2DM+NS), low-dose (100 mg/kg) Met-treated diabetic group (T2DM+M1), middle-dose (300 mg/kg) Met-treated diabetic group (T2DM+M2), high-dose (500 mg/kg) Met-treated diabetic group (T2DM+M3). Results are expressed as Mean ± SEM of three independent experiments. Scale bars = 100 μm, #p < 0.05 versus Ctrl group; ∗p < 0.05 versus T2DM+NS group.
4). Cai's herbal tea enhances mitochondrial autophagy of type 1 diabetic mellitus β cells through the AMPK/mTOR pathway and alleviates inflammatory response. Acta diabetologica, 2024 (PubMed: 38954041) [IF=3.8]
5). Mechanism of ischemic brain injury repair by endothelial progenitor cell‑derived exosomes. Molecular Medicine Reports, 2022 (PubMed: 35775379) [IF=3.4]
6). Assessment of antidiabetic effect of 4-HIL in type 2 diabetic and healthy Sprague Dawley rats. HUMAN & EXPERIMENTAL TOXICOLOGY, 2022 (PubMed: 35072544) [IF=2.8]
7). Experimental Study on the Effect of Tibetan Medicine Triphala on the Proliferation and Apoptosis of Pancreatic Islet β Cells through Incretin-cAMP Signaling Pathway. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2020 (PubMed: 31813891) [IF=2.0]

Application: WB    Species: rat    Sample: pancreas

Fig. 4.| Western blot was used to detect the expression of related proteins in rat pancreas.(a) Compared with the model group, the high-dose group of THL can reduce the expression of TXNIP in pancreatic tissue of diabetic Wistar rats by STZ modeling (p<0.01). (b, d, e, f) Compared with the model group, THL can up-regulate the expression of GLP-1R, P-PKA, AKT and insulin in rat pancreatic tissues (p<0.01). (c) For PKA, compared with the model group, there was significant difference between the low dose THL group (p < 0. 05).

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