Insulin Antibody - #AF5109

>>买3送1 进行中>>
(5)   (6)  
产品: Insulin 抗体
货号: AF5109
描述: Rabbit polyclonal antibody to Insulin
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 11 kDa; 12kD(Calculated).
蛋白号: P01308
RRID: AB_2837595

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(80%), Sheep(80%), Rabbit(80%), Dog(80%)
克隆:
Polyclonal
特异性:
Insulin Antibody detects endogenous levels of total Insulin.
RRID:
AB_2837595
引用格式: Affinity Biosciences Cat# AF5109, RRID:AB_2837595.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IDDM; IDDM1; IDDM2; ILPR; ins; INS_HUMAN; Insulin A chain; Insulin B chain; IRDN; MODY10; Preproinsulin; Proinsulin; Proinsulin precursor;

抗原和靶标

免疫原:
Uniprot:

P01308(INS_HUMAN) >>Visit HPA database.

基因/基因ID:
INS(3630)
描述:
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
序列:
MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Horse
70
Chicken
60
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01308 作为底物

Site PTM Type Enzyme
S98 Phosphorylation
Y108 Phosphorylation

研究背景

功能:

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

蛋白家族:

Belongs to the insulin family.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). Celastrol targets the ChREBP-TXNIP axis to ameliorates type 2 diabetes mellitus. Phytomedicine (PubMed: 36603341) [IF=7.9]
2). Metformin inhibits MAPK signaling and rescues pancreatic aquaporin 7 expression to induce insulin secretion in type 2 diabetes mellitus. Journal of Biological Chemistry (PubMed: 34303707) [IF=4.8]

Application: IF/ICC    Species: Rat    Sample: INS-1 cells

Figure 1 AQP7 expression in pancreatic islets. Double immunofluorescent staining for AQP7 in red and insulin (a marker of pancreatic β-cell) in green showed AQP7 was colocalized with insulin in yellow. Arrows show AQP7 detectable in pancreatic β-cell marker. Nuclei are labeled by DAPI in blue.A, bar graph shows a quantification of insulin fluorescence. B, bar graph shows a quantification of AQP7 fluorescence. Control group (Ctrl), untreated diabetic group (T2DM), 0.9% normal saline-treated diabetic group (T2DM+NS), low-dose (100 mg/kg) Met-treated diabetic group (T2DM+M1), middle-dose (300 mg/kg) Met-treated diabetic group (T2DM+M2), high-dose (500 mg/kg) Met-treated diabetic group (T2DM+M3). Results are expressed as Mean ± SEM of three independent experiments. Scale bars = 100 μm, #p < 0.05 versus Ctrl group; ∗p < 0.05 versus T2DM+NS group.
3). Mechanism of ischemic brain injury repair by endothelial progenitor cell‑derived exosomes. Molecular Medicine Reports (PubMed: 35775379) [IF=3.4]
4). Assessment of antidiabetic effect of 4-HIL in type 2 diabetic and healthy Sprague Dawley rats. HUMAN & EXPERIMENTAL TOXICOLOGY (PubMed: 35072544) [IF=2.8]
5). Experimental Study on the Effect of Tibetan Medicine Triphala on the Proliferation and Apoptosis of Pancreatic Islet β Cells through Incretin-cAMP Signaling Pathway. BIOLOGICAL & PHARMACEUTICAL BULLETIN (PubMed: 31813891) [IF=2.0]

Application: WB    Species: rat    Sample: pancreas

Fig. 4.| Western blot was used to detect the expression of related proteins in rat pancreas.(a) Compared with the model group, the high-dose group of THL can reduce the expression of TXNIP in pancreatic tissue of diabetic Wistar rats by STZ modeling (p<0.01). (b, d, e, f) Compared with the model group, THL can up-regulate the expression of GLP-1R, P-PKA, AKT and insulin in rat pancreatic tissues (p<0.01). (c) For PKA, compared with the model group, there was significant difference between the low dose THL group (p < 0. 05).

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.