| 产品: | Smad9 抗体 |
| 货号: | AF5114 |
| 描述: | Rabbit polyclonal antibody to Smad9 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Sheep, Chicken |
| 分子量: | 52 kDa; 52kD(Calculated). |
| 蛋白号: | O15198 |
| RRID: | AB_2837600 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5114, RRID:AB_2837600.
展开/折叠
MAD homolog 9; Madh6; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD, mothers against DPP homolog 9 (Drosophila); SMAD8A; SMAD8B; Smad9; SMAD9_HUMAN;
抗原和靶标
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
- O15198 SMAD9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYPHSPGSPSEPESPYQHSVDTPPLPYHATEASETQSGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15198 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K25 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K159 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot |
研究背景
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Cytoplasm. Nucleus.
Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit. Interacts with RANBP3L.
Belongs to the dwarfin/SMAD family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
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