产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5118, RRID:AB_2837604.
展开/折叠
Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3;
抗原和靶标
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
- P55210 CASP7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADDQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEEDHTNAACFACILLSHGEENVIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ
翻译修饰 - P55210 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K38 | Methylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| T173 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| K177 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Q13177 (PAK2) | Uniprot |
研究背景
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
Cytoplasm.
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Interacts with ATXN3 (short isoform 1).
Belongs to the peptidase C14A family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
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