产品: IL5 抗体
货号: AF5123
描述: Rabbit polyclonal antibody to IL5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Rabbit
分子量: 15 kDa; 15kD(Calculated).
蛋白号: P05113
RRID: AB_2837609

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Rabbit(89%)
克隆:
Polyclonal
特异性:
IL5 Antibody detects endogenous levels of total IL5.
RRID:
AB_2837609
引用格式: Affinity Biosciences Cat# AF5123, RRID:AB_2837609.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; IL-5; IL5; IL5_HUMAN; Interleukin 5 (colony stimulating factor, eosinophil); Interleukin 5; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; TRF;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
序列:
MRMLLHLSLLALGAAYVYAIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
89
Pig
78
Horse
78
Bovine
78
Sheep
78
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05113 作为底物

Site PTM Type Enzyme
T22 O-Glycosylation
N47 N-Glycosylation
T73 Phosphorylation

研究背景

功能:

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer; disulfide-linked.

蛋白家族:

Belongs to the IL-5 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Asthma.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Allograft rejection.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

文献引用

1). Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheep. Frontiers in Immunology, 2023 (PubMed: 37275885) [IF=7.3]

Application: WB    Species: Hu Sheep    Sample: liver tissue

Figure 8 Effect of DF supplementation on mitophagy. (A) Expression of autophagy-related genes. (B, C) Expression of autophagy-related proteins in the whole cell lysate. (D, E) Expression of mitophagy-related proteins in the mitochondria protein extract. n=6 per group and the values are the mean ± SEM. ** P

2). Pongamol Alleviates Neuroinflammation and Promotes Autophagy in Alzheimer's Disease by Regulating the Akt/mTOR Signaling Pathway. Journal of agricultural and food chemistry, 2024 (PubMed: 38841893) [IF=6.1]

3). Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet. Journal of Dairy Science, 2023 (PubMed: 36631320) [IF=3.5]

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