CD57 Antibody - #AF5125

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产品: CD57 抗体
货号: AF5125
描述: Rabbit polyclonal antibody to CD57
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Horse, Dog
分子量: 38 kDa; 38kD(Calculated).
蛋白号: Q9P2W7
RRID: AB_2837611

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Horse(82%), Dog(%)
克隆:
Polyclonal
特异性:
CD57 Antibody detects endogenous levels of total CD57.
RRID:
AB_2837611
引用格式: Affinity Biosciences Cat# AF5125, RRID:AB_2837611.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; B3gat1; Beta 1 3 glucuronyltransferase 1; Beta-1; Beta-1,3-glucuronyltransferase 1; CD 57; CD57; CD57 antigen; Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU 7; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1;

抗原和靶标

免疫原:
Uniprot:

Q9P2W7(B3GA1_HUMAN) >>Visit HPA database.

基因/基因ID:
B3GAT1(27087)
表达:
Q9P2W7 B3GA1_HUMAN:

Mainly expressed in the brain.

描述:
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin.
序列:
MPKRRDILAIVLIVLPWTLLITVWHQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
82
Dog
80
Bovine
70
Sheep
70
Chicken
64
Rabbit
64
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9P2W7 作为底物

Site PTM Type Enzyme
Y282 Phosphorylation

研究背景

功能:

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

翻译修饰:

The soluble form derives from the membrane form by proteolytic processing.

细胞定位:

Golgi apparatus membrane>Single-pass type II membrane protein. Secreted.

Golgi apparatus membrane>Single-pass type II membrane protein. Endoplasmic reticulum membrane. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in the brain.

亚基结构:

Homodimer. Interacts with SAR1A.

蛋白家族:

Belongs to the glycosyltransferase 43 family.

研究领域

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Frontiers in Immunology, 2022 (PubMed: 35350787) [IF=5.7]

Application: IHC    Species: Mice    Sample: bronchoalveolar lavage fluid (BRLF) and lung tissues

Figure 8 Immune cell infiltration in COPD mouse models. (A) Body weight of mice. (B) The mean alveolar septal thickness (MAST). (C) Mean linear intercept (MLI). (D) Destructive index (DI). (E) Representative HE staining images (100x and 400x). (F) The proportion of immune cells. (G) The expression of IL-6 in bronchoalveolar lavage fluid (BRLF) and lung tissues of mice. (H) The expression of IL-1β in BRLF and lung tissues of mice. (I) The expression of TNF-α in BRLF and lung tissues of mice. (J) Representative immunohistochemistry images of CD8a and CD57 (400x). (K) Representative immunofluorescence images of CD19 and CD20 (400x). For panel G, H, and I, the unit for BRLF is pg/mL, and the unit for lung tissue is pg/mg. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control.

Application: IHC    Species: mouse    Sample: lung

FIGURE 8 | Immune cell infiltration in COPD mouse models.(J) Representative immunohistochemistry images of CD8a and CD57 (400x).

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