Caspase 4 Antibody - #AF5130

Fig. 7. The oxidative stress and inflammation levels in kidney before and after treatment of gold clusters. (A) Schematic diagram of AKI. (B) Serum CREA and BUN levels of mice at 6, 24, and 72 hours with or without gold cluster treatment (n = 3 per group). (C)Representative H&E staining in kidneys on day 3 after injury.(D) Levels of H2O2, MDA, SOD, and GSH/GSSG in kidneys at 6, 24, and 72 hours with or without cluster intervention (n = 3 per group). (E and F) Representative IL-6 and IL-1β staining in kidneys on day 3 after injury. (G) Levels of IL-6, IL-1β, CRP, G-CSF, KC, and MCP-1 in kidneys at 6, 24, and 72 hours with or without cluster intervention (n = 3 per group). (H) Representative terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining in kidneys on day 3 after injury. (I to L) Relative quantification of H&E, IL-6, IL-1β, and TUNEL. Data are presented as means ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the control group and AKI group, analyzed by ANOVA.

Figure 4 Knockdown of NOX4 ameliorates neuronal pyroptosis through caspase 1/GSDMD-N and caspase4/11/GSDMD-C pathways after ICH. (A) Western blot analysis of Procaspase4/11, Cleaved caspase4/11 and Procaspase1 expression in the indicated groups. (B) Western blot analysis of GSDMD-FL, GSDMD-N and GSDMD-C expression in the indicated groups. (C–H) Quantitative analysis of Procaspase4/11 (C), Cleaved caspase4/11 (D), Procaspase1 (E), GSDMD-FL (F), GSDMD-N (G) and GSDMD-C (H) protein levels shown in A and B (n = 4 per group). (I) Representative immunofluorescence images showing the immunoreactivity of GSDMD (red) in neurons (NeuN-positive cells, yellow) in the indicated groups (AAV, GFP, green). The expression of GSDMD in the ICH and ICH + AAV-CON groups were increased, while that in the ICH + AAV-NOX4 group was decreased. Scale bars: 20 µm. (J) Representative immunohistochemical images illustrate the changes in the GSDMD protein expression in the indicated groups. The expression of GSDMD in the ICH and ICH + AAV-CON groups was increased, while that in the ICH + AAV-NOX4 group was decreased. Scale bars: 50 µm. (K, L) Percentage of GSDMD-positive cells in immunofluorescence (K) and immunohistochemical (L) images (n = 5 per group). Data are expressed as the mean ± SEM. ***P < 0.001 (one-way analysis of variance with Dunnett’s multiple comparison test). AAV-CON: AAV9-U6-shRNA (Scramble)-CMV-GFP; AAV-NOX4: AAV9-U6-shRNA (NOX4)-CMV-GFP; GSDMD: gasdermin D; ICH: intracerebral hemorrhage; MRI: magnetic resonance imaging; NOX4: nicotinamide adenine dinucleotide phosphate oxidase 4.

Fig. 2. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Caspase-11 expression in liver tissues was detected by Western biotting. B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.

Fig. 3. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Representative fluorescence image of caspase-11 expression from liver sections(200x). B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.