| 产品: | E2F5 抗体 |
| 货号: | AF5137 |
| 描述: | Rabbit polyclonal antibody to E2F5 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 37 kDa; 38kD(Calculated). |
| 蛋白号: | Q15329 |
| RRID: | AB_2837623 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5137, RRID:AB_2837623.
展开/折叠
E2F 5; E2F transcription factor 5; E2F transcription factor 5 p130 binding; E2F-5; E2f5; E2F5_HUMAN; p130 binding; Transcription factor E2F5;
抗原和靶标
- Q15329 E2F5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAEPASSGQQAPAGQGQGQRPPPQPPQAQAPQPPPPPQLGGAGGGSSRHEKSLGLLTTKFVSLLQEAKDGVLDLKAAADTLAVRQKRRIYDITNVLEGIDLIEKKSKNSIQWKGVGAGCNTKEVIDRLRYLKAEIEDLELKERELDQQKLWLQQSIKNVMDDSINNRFSYVTHEDICNCFNGDTLLAIQAPSGTQLEVPIPEMGQNGQKKYQINLKSHSGPIHVLLINKESSSSKPVVFPVPPPDDLTQPSSQSLTPVTPQKSSMATQNLPEQHVSERSQALQQTSATDISSAGSISGDIIDELMSSDVFPLLRLSPTPADDYNFNLDDNEGVCDLFDVQILNY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15329 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K53 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Nucleus.
Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.
Belongs to the E2F/DP family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
限制条款
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