(3)
(2)
| 产品: | BBC3 抗体 |
| 货号: | AF5173 |
| 描述: | Rabbit polyclonal antibody to BBC3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Dog |
| 分子量: | 20 kDa; 21kD(Calculated). |
| 蛋白号: | Q9BXH1 |
| RRID: | AB_2837659 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5173, RRID:AB_2837659.
展开/折叠
BBC 3; Bbc3; BBC3_HUMAN; BCL 2 binding component 3; Bcl-2-binding component 3; BCL2 binding component 3; JFY 1; JFY-1; JFY1; p53 up regulated modulator of apoptosis; p53 up-regulated modulator of apoptosis; p53 Upregulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1;
抗原和靶标
A synthesized peptide derived from human BBC3(PUMA), corresponding to a region within the internal amino acids.
- Q9BXH1 BBC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARARQEGSSPEPVEGLARDGPRPFPLGRLVPSAVSCGLCEPGLAAAPAAPTLLPAAYLCAPTAPPAVTAALGGSRWPGGPRSRPRGPRPDGPQPSLSLAEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BXH1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
| Y58 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S96 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S166 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | P04626 (ERBB2) | Uniprot |
研究背景
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
Mitochondrion.
Note: Localized to the mitochondria in order to induce cytochrome c release.
Ubiquitously expressed.
Interacts with MCL1 and BCL2A1 (By similarity). Interacts (via BH3 domain) with BCL2. Interacts with BCL2L1/BCL-XL. Interacts (via BH3 domain) with NOL3/ARC (via CARD domain); this interaction prevents BBC3 association with BCL2 and results in CASP8 activation (By similarity).
The BH3 motif is intrinsically disordered.
Belongs to the Bcl-2 family.
研究领域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
文献引用
Application: WB Species: human Sample:
限制条款
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