(2)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5188, RRID:AB_2837674.
展开/折叠
Acute myeloid leukemia 2 protein; Acute myeloid leukemia gene 2; AML 2; AML2; CBF alpha 3; CBF-alpha-3; CBFA 3; CBFA3; Core binding factor alpha 3 subunit; core binding factor; Core binding factor runt domain alpha subunit 3; Core binding factor subunit alpha 3; core-binding factor; Core-binding factor subunit alpha-3; Oncogene AML 2; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Pebp2a3; PEBP2aC; Polyomavirus enhancer binding protein 2 alpha C subunit; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain alpha subunit 3; runt related transcription factor 3; Runt-related transcription factor 3; RUNX 3; Runx3; RUNX3_HUMAN; SL3 3 enhancer factor 1 alpha C subunit; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core binding factor alpha C subunit; SL3/AKV core-binding factor alpha C subunit; Transcription factor AML2;
抗原和靶标
- Q13761 RUNX3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRIPVDPSTSRRFTPPSPAFPCGGGGGKMGENSGALSAQAAVGPGGRARPEVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKLEDQTKPFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTSELNPFSDPRQFDRSFPTLPTLTESRFPDPRMHYPGAMSAAFPYSATPSGTSISSLSVAGMPATSRFHHTYLPPPYPGAPQNQSGPFQANPSPYHLYYGTSSGSYQFSMVAGSSSGGDRSPTRMLASCTSSAASVAAGNLMNPSLGGQSDGVEADGSHSNSPTALSTPGRMDEAVWRPY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13761 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T14 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | P11309 (PIM1) | Uniprot |
| T151 | Phosphorylation | P11309 (PIM1) | Uniprot |
| T153 | Phosphorylation | P11309 (PIM1) | Uniprot |
| T155 | Phosphorylation | P11309 (PIM1) | Uniprot |
| K171 | Acetylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Sumoylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| T212 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| R216 | Methylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| R262 | Methylation | Uniprot | |
| R267 | Methylation | Uniprot | |
| R302 | Methylation | Uniprot | |
| S356 | Phosphorylation | P11802 (CDK4) | Uniprot |
研究背景
Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Nucleus. Cytoplasm.
Note: The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation.
Expressed in gastric cancer tissues (at protein level).
Heterodimer with CBFB. RUNX3 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization (By similarity). Interacts with TLE1 and SUV39H1and. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Interacts with FOXP3. Interacts with ZFHX3. Interacts with TBX21 (By similarity).
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
研究领域
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
文献引用
Application: WB Species: human Sample:
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