(1)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5207, RRID:AB_2837693.
展开/折叠
ECS-1; ECS1; Epidermal surface antigen 1; Epidermal surface antigen; ESA; ESA1; Flot2; FLOT2_HUMAN; Flotillin 2 (epidermal surface antigen 1); Flotillin-2; M17S1; Membrane component chromosome 17 surface marker 1; Membrane component chromosome 17 surface marker 1 homolog; membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1); Membrane component, chromosome 17, surface marker 1; REG-1; Reggie-1; Reggie-2;
抗原和靶标
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
- Q14254 FLOT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNCHTVGPNEALVVSGGCCGSDYKQYVFGGWAWAWWCISDTQRISLEIMTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEILRTDKELIATVRRPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEAYQKYGDAAKMALVLEALPQIAAKIAAPLTKVDEIVVLSGDNSKVTSEVNRLLAELPASVHALTGVDLSKIPLIKKATGVQV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14254 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| R127 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y163 | Phosphorylation | P06241 (FYN) | Uniprot |
| S166 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot |
研究背景
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.
Cell membrane>Peripheral membrane protein. Membrane>Caveola>Peripheral membrane protein. Endosome. Membrane>Lipid-anchor.
Note: Membrane-associated protein of caveolae.
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.
Belongs to the band 7/mec-2 family. Flotillin subfamily.
研究领域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
文献引用
Application: WB Species: Hu Sheep Sample: liver tissue
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