Claudin 6 Antibody - #AF5213

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产品: Claudin 6 抗体
货号: AF5213
描述: Rabbit polyclonal antibody to Claudin 6
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 23 kDa; 23kD(Calculated).
蛋白号: P56747
RRID: AB_2837699

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)
克隆:
Polyclonal
特异性:
Claudin 6 Antibody detects endogenous levels of total Claudin 6.
RRID:
AB_2837699
引用格式: Affinity Biosciences Cat# AF5213, RRID:AB_2837699.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Claudin-6; Claudin6; CLD6_HUMAN; CLDN 6; CLDN6; OTTHUMP00000159248; Skullin 2; Skullin; UNQ757/PRO1488;

抗原和靶标

免疫原:
Uniprot:

P56747(CLD6_HUMAN) >>Visit HPA database.

基因/基因ID:
CLDN6(9074)
表达:
P56747 CLD6_HUMAN:

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

描述:
Plays a major role in tight junction-specific obliteration of the intercellular space.
序列:
MASAGMQILGVVLTLLGWVNGLVSCALPMWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLVALFGLLVYLAGAKCTTCVEEKDSKARLVLTSGIVFVISGVLTLIPVCWTAHAIIRDFYNPLVAEAQKRELGASLYLGWAASGLLLLGGGLLCCTCPSGGSQGPSHYMARYSTSAPAISRGPSEYPTKNYV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
88
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Pig
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P56747 作为底物

Site PTM Type Enzyme
Y200 Phosphorylation
S201 Phosphorylation
T202 Phosphorylation
S203 Phosphorylation
S208 Phosphorylation
S212 Phosphorylation
Y214 Phosphorylation
T216 Phosphorylation
K217 Ubiquitination
Y219 Phosphorylation

研究背景

功能:

Plays a major role in tight junction-specific obliteration of the intercellular space.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

亚基结构:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with CLDN1, CD81 and OCLN.

蛋白家族:

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Downregulation of CLDN6 inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma. Translational cancer research (PubMed: 37588735) [IF=0.9]

Application: IF/ICC    Species: Human    Sample: SMMC-7721 and MHCC-97H cells

Figure 1 Transfection of SMMC-7721 and MHCC-97H cells with CLDN6-siRNA or NC-siRNA for 48 h. (A,B) Detection of CLDN6 expression by qRT-PCR, ***, P

Application: WB    Species: Human    Sample: SMMC-7721 and MHCC-97H cells

Figure 4 Induction of apoptosis via downregulation of JAK2/STAT3 signaling pathway activation by CLDN6 silencing. Transfection of MMC-7721 and MHCC-97H cells with CLDN6-siRNA or NC-siRNA sequence. (A,B) Attenuation in p-JAK2, p-STAT3, p-JAK2/JAK2, p-STAT3/STAT3, CLDN6 and Bcl-2 expression and enhancement of Bax expression and cleaved caspase-3 levels via CLDN6 silencing (*, P
2). Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma. STAR Protocols (PubMed: 36595904)

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