| 产品: | TDG 抗体 |
| 货号: | AF5214 |
| 描述: | Rabbit polyclonal antibody to TDG |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 46 kDa; 46kD(Calculated). |
| 蛋白号: | Q13569 |
| RRID: | AB_2837700 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5214, RRID:AB_2837700.
展开/折叠
C JUN leucine zipper interactive protein; C JUN leucine zipper interactive protein; C-JUN leucine zipper interactive protein JZA-3; E130317C12Rik; EC 3.2.2.29; G/T mismatch specific thymine DNA glycosylase; G/T mismatch specific thymine DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; JZA 3; Jza1; T:G mismatch thymine glycosylase; Tdg; TDG_HUMAN; Thymine DNA glycosylase; Thymine-DNA glycosylase;
抗原和靶标
- Q13569 TDG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPAQEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCGTQEQEEESHA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13569 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K71 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K84 | Acetylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K248 | Sumoylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K330 | Sumoylation | Uniprot |
研究背景
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
Nucleus.
Homodimer. Interacts with AICDA and GADD45A.
Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
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