(3)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5218, RRID:AB_2837704.
展开/折叠
High mobility group (nonhistone chromosomal) protein isoforms I and Y; High mobility group AT hook 1; High mobility group AT-hook protein 1; High mobility group protein 1; High mobility group protein A1; High mobility group protein HMG-I/HMG-Y; High mobility group protein Ia; High mobility group protein R; HMG R; HMG-I(Y); HMGA1; HMGA1_HUMAN; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; Nonhistone chromosomal high mobility group protein HMG I/HMG Y;
抗原和靶标
- P17096 HMGA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17096 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| K7 | Methylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Methylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K18 | Acetylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Methylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| R26 | Methylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K31 | Methylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) , Q9H2X6 (HIPK2) | Uniprot |
| T39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K46 | Acetylation | Uniprot | |
| K46 | Methylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Q9H2X6 (HIPK2) , P06493 (CDK1) | Uniprot |
| K55 | Acetylation | Uniprot | |
| K55 | Methylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| R58 | Methylation | Uniprot | |
| R60 | Methylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Methylation | Uniprot | |
| S64 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K65 | Acetylation | Uniprot | |
| K65 | Methylation | Uniprot | |
| K65 | Sumoylation | Uniprot | |
| K67 | Acetylation | Uniprot | |
| K67 | Methylation | Uniprot | |
| K71 | Acetylation | Uniprot | |
| K71 | Methylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| R73 | Methylation | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K74 | Methylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | P06493 (CDK1) , Q9H2X6 (HIPK2) | Uniprot |
| R81 | Methylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| R84 | Methylation | Uniprot | |
| R86 | Methylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot |
研究背景
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Nucleus. Chromosome.
Interacts with HIPK2 (By similarity).
Belongs to the HMGA family.
文献引用
Application: WB Species: Human Sample:
Application: WB Species: Human Sample: OVCAR-3 cells
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