产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5223, RRID:AB_2837709.
展开/折叠
JTK 1; JTK1; Non receptor tyrosine protein kinase 2; Non receptor tyrosine protein kinase TYK2; Non-receptor tyrosine-protein kinase TYK2; OTTHUMP00000232745; OTTHUMP00000232746; OTTHUMP00000232748; Protein Tyrosine Kinase 2; TYK 2; Tyk2; TYK2_HUMAN; Tyrosine kinase 2;
抗原和靶标
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
- P29597 TYK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLRHWGMARGSKPVGDGAQPMAAMGGLKVLLHWAGPGGGEPWVTFSESSLTAEEVCIHIAHKVGITPPCFNLFALFDAQAQVWLPPNHILEIPRDASLMLYFRIRFYFRNWHGMNPREPAVYRCGPPGTEASSDQTAQGMQLLDPASFEYLFEQGKHEFVNDVASLWELSTEEEIHHFKNESLGMAFLHLCHLALRHGIPLEEVAKKTSFKDCIPRSFRRHIRQHSALTRLRLRNVFRRFLRDFQPGRLSQQMVMVKYLATLERLAPRFGTERVPVCHLRLLAQAEGEPCYIRDSGVAPTDPGPESAAGPPTHEVLVTGTGGIQWWPVEEEVNKEEGSSGSSGRNPQASLFGKKAKAHKAVGQPADRPREPLWAYFCDFRDITHVVLKEHCVSIHRQDNKCLELSLPSRAAALSFVSLVDGYFRLTADSSHYLCHEVAPPRLVMSIRDGIHGPLLEPFVQAKLRPEDGLYLIHWSTSHPYRLILTVAQRSQAPDGMQSLRLRKFPIEQQDGAFVLEGWGRSFPSVRELGAALQGCLLRAGDDCFSLRRCCLPQPGETSNLIIMRGARASPRTLNLSQLSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLADVLTVNPDSPASDPTVFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P29597 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T67 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | Uniprot | |
| Y433 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S613 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| Y827 | Phosphorylation | Uniprot | |
| Y851 | Phosphorylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| K930 | Ubiquitination | Uniprot | |
| K933 | Ubiquitination | Uniprot | |
| K945 | Ubiquitination | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| K1046 | Ubiquitination | Uniprot | |
| Y1054 | Phosphorylation | P29597 (TYK2) , P23458 (JAK1) | Uniprot |
| Y1055 | Phosphorylation | P29597 (TYK2) , P23458 (JAK1) | Uniprot |
| K1074 | Ubiquitination | Uniprot | |
| K1175 | Ubiquitination | Uniprot |
翻译修饰 - P29597 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15304 (SIVA1) | Y53 | Uniprot |
| O15304 (SIVA1) | Y162 | Uniprot |
| P17181 (IFNAR1) | Y466 | Uniprot |
| P17181 (IFNAR1) | Y481 | Uniprot |
| P29597 (TYK2) | Y1054 | Uniprot |
| P29597 (TYK2) | Y1055 | Uniprot |
| P42224-2 (STAT1) | Y701 | Uniprot |
| P51452 (DUSP3) | Y138 | Uniprot |
| P63244 (RACK1) | Y194 | Uniprot |
| Q13651 (IL10RA) | Y446 | Uniprot |
| Q13651 (IL10RA) | Y496 | Uniprot |
| Q9BZL6 (PRKD2) | Y438 | Uniprot |
研究背景
Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
Interacts with JAKMIP1.
The FERM domain mediates interaction with JAKMIP1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
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