产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5225, RRID:AB_2837711.
展开/折叠
CK 14; CK-14; ck14; Cytokeratin 14; Cytokeratin-14; Cytokeratin14; Dowling Meara; EBS3; EBS4; Epidermolysis bullosa simplex; K14; K1C14_HUMAN; Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); Keratin 14; Keratin; Keratin type I cytoskeletal 14; Keratin, type I cytoskeletal 14; Keratin-14; Keratin14; Koebner; Krt 14; Krt14; NFJ; OTTHUMP00000164624; type I cytoskeletal 14;
抗原和靶标
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
- P02533 K1C14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P02533 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T9 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| K15 | Methylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K243 | Methylation | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| K250 | Sumoylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| Y398 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| T414 | Phosphorylation | Uniprot | |
| Y415 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot |
研究背景
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Cytoplasm. Nucleus.
Note: Expressed in both as a filamentous pattern.
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Heterotetramer of two type I and two type II keratins. disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Interacts with EPPK1 (By similarity). Interacts with KLHL24.
Belongs to the intermediate filament family.
研究领域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
限制条款
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