FSHR Antibody - #AF5242

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产品: FSHR 抗体
货号: AF5242
描述: Rabbit polyclonal antibody to FSHR
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 78 kDa; 78kD(Calculated).
蛋白号: P23945
RRID: AB_2837728

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 50ul RMB¥ 1250 现货
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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%)
克隆:
Polyclonal
特异性:
FSHR Antibody detects endogenous levels of total FSHR.
RRID:
AB_2837728
引用格式: Affinity Biosciences Cat# AF5242, RRID:AB_2837728.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Follicle stimulating hormone receptor; Follicle stimulating hormone receptor isoform 1; Follicle-stimulating hormone receptor; Follitropin receptor; FSH receptor; FSH-R; Fshr; FSHR_HUMAN; FSHRO; LGR1; MGC141667; MGC141668; ODG1; ovarian dysgenesis 1;

抗原和靶标

免疫原:
Uniprot:

P23945(FSHR_HUMAN) >>Visit HPA database.

基因/基因ID:
FSHR(2492)
表达:
P23945 FSHR_HUMAN:

Sertoli cells and ovarian granulosa cells.

描述:
Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
序列:
MALLLVSLLAFLSLGSGCHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFGDLEKIEISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHKIHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELPNDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPTLEKLVALMEASLTYPSHCCAFANWRRQISELHPICNKSILRQEVDYMTQARGQRSSLAEDNESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKPDAFNPCEDIMGYNILRVLIWFISILAITGNIIVLVILTTSQYKLTVPRFLMCNLAFADLCIGIYLLLIASVDIHTKSQYHNYAIDWQTGAGCDAAGFFTVFASELSVYTLTAITLERWHTITHAMQLDCKVQLRHAASVMVMGWIFAFAAALFPIFGISSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHIYLTVRNPNIVSSSSDTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPINSCANPFLYAIFTKNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPRVTNGSTYILVPLSHLAQN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
70
Xenopus
50
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

翻译修饰:

Sulfated.

N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Sertoli cells and ovarian granulosa cells.

亚基结构:

Homotrimer. Functions as a homotrimer binding the FSH hormone heterodimer composed of CGA and FSHB. Interacts with ARRB2 (By similarity). Interacts with APPL2; interaction is independent of follicle stimulating hormone stimulation.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

文献引用

1). A novel nuclear receptor NR1D1 suppresses HSD17B12 transcription to regulate granulosa cell apoptosis and autophagy via the AMPK pathway in sheep. International journal of biological macromolecules, 2025 (PubMed: 39986531) [IF=7.7]
2). Di-(2-ethylhexyl) phthalate exposure induces ferroptosis by regulating the Nrf2-mediated signaling pathway in mouse ovaries. Ecotoxicology and environmental safety, 2024 (PubMed: 39321527) [IF=6.2]
3). CCDC134 enhances ovarian reserve function and angiogenesis by directly interacting with INHA in a mouse model of premature ovarian insufficiency. Apoptosis : an international journal on programmed cell death, 2025 (PubMed: 40042746) [IF=6.1]
4). Study on the effects and mechanisms of Wenzhong Bushen Formula in improving ovarian reserve decline in mice based on network pharmacology. Journal of ethnopharmacology, 2024 (PubMed: 38218503) [IF=4.8]
5). Effects of icariin as a feed additive on the reproductive function in bucks (Capra hircus). Frontiers in veterinary science, 2024 (PubMed: 39575435) [IF=3.2]

Application: IHC    Species: goat    Sample:

Figure 4. Administration of ICA to dairy goats increased the expression of hormone receptors and testosterone synthesis in the testes. (A) Immunohistochemistry analyzes the distribution of hormonal receptors in the testes. (a) The distribution of LHR in the control group. (b) The distribution of LHR in the ICA group. (c) The distribution of FSHR in the control group. (d) The distribution of FSHR in the ICA group. (e) The distribution of AR in the control group. (f) The distribution of AR in the ICA group. (g) The distribution of 3β-HSD in the control group. (h) The distribution of 3β-HSD in the ICA group. ST, seminiferous tubules; Lu, lumen; Curved arrow, Sertoli cells; Arrow, Leydig cells; Scale bar = 100 μm. (B) qPCR and western blot analyze the mRNA and protein expression in the testes. (a) The mRNA levels of AR, LHR, and FSHR were examined by qPCR. (b) The mRNA levels of steroidogenesis enzymes were examined by qPCR. (c) Western blot analysis was performed to measure the expression of target proteins. β-actin was utilized as a loading control. (d,e) The histogram represents the quantification of proteins levels. Each value represents the mean ± SEM, *p 
6). Elevated RBP4 in Subclinical Ketosis Cows Inhibits Follicular Granulosa Cell Proliferation and Steroid Hormone Synthesis. Animals : an open access journal from MDPI, 2024 (PubMed: 39518840) [IF=3.0]
7). Resveratrol Promotes Proliferation, Antioxidant Properties, and Progesterone Production in Yak (Bos grunniens) Granulosa Cells. Animals : an open access journal from MDPI, 2024 (PubMed: 38254409) [IF=2.7]

Application: IF/ICC    Species: Yak    Sample: GCs

Figure 1 Identification of yak follicular granulosa cells via FSHR immunofluorescence. Second-generation cells were used in the experiment. Red fluorescence indicates the specific expression of FSHR, and blue fluorescence indicates the nucleus. Bar = 20 μm.
8). Regulation of proliferation, apoptosis, hormone secretion and gene expression by acetyl-L-carnitine in yak (Bos grunniens) granulosa cells. Theriogenology, 2023 (PubMed: 36972666) [IF=2.4]
9). Role of secreted frizzled-related protein 5 in granulosa cells of hu sheep ovaries. Theriogenology, 2024 (PubMed: 38805996) [IF=2.4]
10). Exosomes from adipose-derived stem cells alleviate premature ovarian failure via blockage of autophagy and AMPK/mTOR pathway. PeerJ, 2023 (PubMed: 38107591) [IF=2.3]
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