GATA4 Antibody - #AF5245

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产品: GATA4 抗体
货号: AF5245
描述: Rabbit polyclonal antibody to GATA4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 46 kDa; 45kD(Calculated).
蛋白号: P43694
RRID: AB_2837731

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
GATA4 Antibody detects endogenous levels of total GATA4.
RRID:
AB_2837731
引用格式: Affinity Biosciences Cat# AF5245, RRID:AB_2837731.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ASD2; GATA 4; GATA binding protein 4; GATA-binding factor 4; gata4; GATA4_HUMAN; MGC126629; Transcription factor GATA 4; Transcription factor GATA-4; Transcription factor GATA4; VSD1;

抗原和靶标

免疫原:
Uniprot:

P43694(GATA4_HUMAN) >>Visit HPA database.

基因/基因ID:
GATA4(2626)
描述:
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development.
序列:
MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P43694 作为底物

Site PTM Type Enzyme
S105 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P11802 (CDK4)
S109 Phosphorylation
Y236 Phosphorylation
Y244 Phosphorylation
S262 Phosphorylation P17612 (PRKACA)
S264 Phosphorylation
S395 Phosphorylation
S401 Phosphorylation
S406 Phosphorylation
S412 Phosphorylation

研究背景

功能:

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase.

翻译修饰:

Methylation at Lys-300 attenuates transcriptional activity.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts (via the second Zn finger) with NFATC4 (By similarity). Interacts with LMCD1 (By similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro. PLoS One, 2015 (PubMed: 25898012) [IF=3.7]

Application: WB    Species:    Sample: miR-20b cells

Fig 7. |Effects of miR-20b on the BMP signaling pathway, as assessed by by western blotting. a): in the miR-20b overexpression group; b): in miR-20b silenced group. (n = 3, *: P<0.05, **: P<0.01, ***: P<0.001)
2). BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells. Theriogenology, 2023 (PubMed: 36525856) [IF=2.8]
3). Identification of key microRNAs and the underlying molecular mechanism in spinal cord ischemia-reperfusion injury in rats. PeerJ, 2021 (PubMed: 34123589) [IF=2.7]

Application: WB    Species: Rat    Sample: spinal cord tissues

Figure 8 Effects of AMO-3568 on the protein expression levels of RPBJ (A, B), GATA6 (A, C) and GATA4 (A, D) were measured by Western blot assay. n = 4 for per group. Data were analyzed by one-way ANOVA followed by the Tukey’s test. *p < 0.05, versus the sham group, #p < 0.05 versus the SCII group.
4). Effective isolation of Sertoli cells from New Zealand rabbit testis. Journal of Advanced Veterinary and Animal Research, 2021 (PubMed: 34395591) [IF=1.4]
5). Pathological and Immunohistochemical Analysis of Splenic Metastasis of Adrenal Cortical Carcinoma in a Cat. 畜牧兽医学报, 2021

Application: IHC    Species: Cat    Sample:

A. Synaptophysin在肿瘤组织胞质中呈阳性表达，且阳性区域广泛, bar=200 μm；B. Synaptophysin在肿瘤组织胞质中呈阳性表达(黑色箭头)，bar=100 μm；C.S-100在肿瘤细胞中表达为少量细胞阳性反应(黑色箭头)，bar=100 μm；D. GATA4在肿瘤组织细胞核中表达为阳性反应(黑色箭头)，bar=100 μm；E. Pancytokeratin在肿瘤组织中表达为阴性反应，bar=200 μm；F. Vimentin在肿瘤组织中表达为阴性反应，bar=200 μm A. Synaptophysin was positively expressed in the cytoplasm of tumor tissues, and the positive region was extensive bar=200 μm; B. Synaptophysin was positively expressed in the cytoplasm of tumor tissue (black arrow), bar=100 μm; C. S-100 was expressed as a small amount of cell positive reaction in tumor cells (black arrow), bar=100 μm; D. GATA4 was positively expressed in the nucleus of tumor tissues (black arrow), bar=100 μm; E. Pancytokeratin was negatively expressed in tumor tissues, bar=200 μm; F. Vimentin was negative in tumor tissue, bar=200 μm 图 3 免疫组化结果 Fig. 3 Immunohistochemical results
6). Formate assay in body fluids: application in methanol poisoning. Biochemical medicine, 1975 (PubMed: 1)

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