产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5268, RRID:AB_2837754.
展开/折叠
HNRNPA 1; Helix destabilizing protein; Helix-destabilizing protein; Heterogeneous nuclear ribonucleoprotein A1; Heterogeneous nuclear ribonucleoprotein A1B protein; Heterogeneous nuclear ribonucleoprotein B2 protein; Heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; HNRNPA1; HNRPA1; MGC102835; Nuclear ribonucleoprotein particle A1 protein; ROA1_HUMAN; Single strand DNA binding protein UP1; Single strand RNA binding protein; Single-strand RNA-binding protein;
抗原和靶标
- P09651 ROA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09651 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| S4 | Phosphorylation | Q9UBS0 (RPS6KB2) | Uniprot |
| S6 | Phosphorylation | Q9UBS0 (RPS6KB2) | Uniprot |
| K8 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| R14 | Methylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| C43 | S-Nitrosylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| R55 | Methylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T103 | Phosphorylation | Uniprot | |
| K105 | Acetylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K106 | Methylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K113 | Acetylation | Uniprot | |
| K113 | Sumoylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| R122 | Methylation | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K145 | Acetylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Methylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| C175 | S-Nitrosylation | Uniprot | |
| K183 | Sumoylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| R194 | Methylation | Uniprot | |
| R196 | Methylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | P17612 (PRKACA) , P68400 (CSNK2A1) , P31749 (AKT1) | Uniprot |
| R206 | Methylation | Uniprot | |
| R218 | Methylation | Uniprot | |
| R225 | Methylation | Uniprot | |
| R232 | Methylation | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| R265 | Methylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| Y318 | Phosphorylation | Uniprot | |
| K329 | Sumoylation | Uniprot | |
| R336 | Methylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| K350 | Acetylation | Uniprot | |
| K350 | Methylation | Uniprot | |
| K350 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| R352 | Methylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S363 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S364 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S365 | Phosphorylation | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| R370 | Methylation | Uniprot | |
| R371 | Methylation | Uniprot |
研究背景
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May bind to specific miRNA hairpins. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1.
(Microbial infection) May play a role in HCV RNA replication.
(Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated.
Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes (PubMed:17289661).
Cytoplasm.
Note: (Microbial infection) In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
Identified in the spliceosome C complex. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SEPT6. Interacts with C9orf72. Interacts with KHDRBS1. Interacts with UBQLN2.
(Microbial infection) Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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