ZEB2 Antibody - #AF5278

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产品: ZEB2 抗体
货号: AF5278
描述: Rabbit polyclonal antibody to ZEB2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 136 kDa.; 136kD(Calculated).
蛋白号: O60315
RRID: AB_2837764

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
ZEB2 Antibody detects endogenous levels of total ZEB2.
RRID:
AB_2837764
引用格式: Affinity Biosciences Cat# AF5278, RRID:AB_2837764.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ42816; HSPC082; KIAA0569; SIP 1; SIP1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; Zeb2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b;

抗原和靶标

免疫原:
Uniprot:

O60315(ZEB2_HUMAN) >>Visit HPA database.

基因/基因ID:
ZEB2(9839)
描述:
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
序列:
MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPEEMKEDYDTMGPEATIQTAINNGTVKNANCTSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQYSNSRSPSLERSSKPLAPNSNPPTKDSLLPRSPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFSMNPFSAKPLYTALPPQSAFPPATFMPPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Bovine
91
Xenopus
64
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60315 作为底物

Site PTM Type Enzyme
R11 Methylation
T34 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
S64 Phosphorylation
T119 Phosphorylation
S142 Phosphorylation
Y147 Phosphorylation
S162 Phosphorylation
S184 Phosphorylation
T188 Phosphorylation
T254 Phosphorylation
T274 Phosphorylation
T285 Phosphorylation
S305 Phosphorylation
S324 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
S331 Phosphorylation
T350 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S356 Phosphorylation
S358 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
T362 Phosphorylation
K377 Acetylation
K391 Sumoylation
S410 Phosphorylation
S431 Phosphorylation
T470 Phosphorylation
S505 Phosphorylation
K632 Ubiquitination
S641 Phosphorylation
S647 Phosphorylation
S703 Phosphorylation
S705 Phosphorylation
S707 Phosphorylation
S726 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
S778 Phosphorylation
S780 Phosphorylation
T782 Phosphorylation
S784 Phosphorylation
S796 Phosphorylation
T802 Phosphorylation
S805 Phosphorylation
S819 Phosphorylation
S829 Phosphorylation
T837 Phosphorylation
S840 Phosphorylation
S841 Phosphorylation
S843 Phosphorylation
S848 Phosphorylation
S853 Phosphorylation
K866 Sumoylation
S1050 Phosphorylation
S1115 Phosphorylation
T1117 Phosphorylation
S1122 Phosphorylation
S1124 Phosphorylation
S1129 Phosphorylation
S1136 Phosphorylation
S1167 Phosphorylation
T1185 Phosphorylation
S1189 Phosphorylation
S1193 Phosphorylation
S1194 Phosphorylation
S1203 Phosphorylation

研究背景

功能:

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

翻译修饰:

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected (By similarity). Interacts with CBX4 and CTBP1.

蛋白家族:

Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). Fisetin Improves Hyperuricemia-Induced Chronic Kidney Disease via Regulating Gut Microbiota-Mediated Tryptophan Metabolism and Aryl Hydrocarbon Receptor Activation. Journal of Agricultural and Food Chemistry, 2021 [IF=6.1]
2). The regulatory effects of metformin on the [SNAIL/miR-34]:[ZEB/miR-200] system in the epithelial-mesenchymal transition(EMT) for colorectal cancer(CRC). European Journal of Pharmacology, 2018 (PubMed: 30017802) [IF=5.0]

Application: WB    Species: human    Sample: SW480 and HCT116 cells

Fig. 3. |Western-blot for SW480 and HCT116 cells (A)(B) Protein strips and quantitative analysis: significant decreases in the SNAIL1, ZEB1 and vimentin and a significant increase in the E-cadherin of metformin and TGF-β-treated samples compared with only TGF-β-treated samples for both SW480 and HCT116 cells; a significant increase in the ZEB2 of metformin and TGF-β-treated samples compared with only TGF-β-treated samples for SW480 cells; significant decreases in the SNAIL2, ZEB1 and ZEB2 of metformin-treated samples compared with negative control samples for HCT116 cells. *P<0.05
3). Network pharmacology- and cell-based assessments identify the FAK/Src pathway as a molecular target for the antimetastatic effect of momordin Ic against cholangiocarcinoma. Heliyon, 2024 (PubMed: 38961933) [IF=4.0]

Application: WB    Species: Human    Sample: KKU-452 cells

Fig. 5 Effects of MIc on EMT in CCA cells. KKU-452 cells were chemically induced to undergo EMT (iEMT). (A) The effect of MIc on cell morphology at concentrations of 1 and 2.5 μM was determined. EMT marker expression, including N-cadherin, vimentin, ZEB2, and FOXC1/2, was further evaluated by (B) Western blot (Supplementary Fig. S1 Images of original blot 5B) and (C) immunocytofluorescence analyses. The data shown are representative of two reproducible experiments.
4). Estradiol promotes EMT in endometriosis via MALAT1/miR200s sponge function. REPRODUCTION, 2019 (PubMed: 30500775) [IF=3.8]

Application: WB    Species: human    Sample: EECs

Figure 2.| (A and B) Expression levels of EMT-associated markers (Zeb1, Zeb2, E-cadherin and Vimentin) with E2 treatment for different doses (0, 10−12, 10−10, 10−8, 10−6 mol/L) and time (0, 24, 48 or 72 h) in EECs detected by Western blot.(M: mol/L); (C and D) Expression levels of MALAT1 and miR200s under E2 for various doses (0, 10−12, 10−10, 10−8, 10−6 mol/L) for 24 h in EECs measured by qRT-PCR. Data were evaluated by one-way ANOVA analysis (*P < 0.05, **P < 0.01, ***P < 0.001 compared with untreated group).
5). Wnt/PCP-YAP-BIRC2 axis maintains cartilage stem/progenitor cell homeostasis in osteoarthritis. Research Square, 2022

Application: WB    Species: Human    Sample: CSPCs

Figure 7. Transcriptomic analysis. a KEGG and GO enrichment analysis of DEGs. b The detailed DEGs that enriched in cell cycle, cellular senescence, autophagy, apoptosis, Hippo signaling pathway, and TNF signaling pathways. c The direct downstream target genes of YAP. d The protein level of Birc2, Snai2, Zenb2, and Ccnd2 in CSPCs.
6). Exosomal miRNA-215-5p Derived from Adipose-Derived Stem Cells Attenuates Epithelial–Mesenchymal Transition of Podocytes by Inhibiting ZEB2. BioMed Research International, 2020 (PubMed: 32149094)

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 5: |The correlation between miR-215-5p and ZEB2 in podocyte migration. (a) Targeted binding sites between miR-215-5p and ZEB2-3′-UTR sequences were predicted by TargetScan database and effect of miR-215-5p on transcription activity of ZEB2 determined by luciferase report assay in 293 cells. (b) The expression of ZEB2 was measured by qRT-PCR in 293 cells treated with miR-215-5p mimic.(c) The protein level of ZEB2 was measured by western blotting in MPC5 cells treated with miR-215-5p mimic

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