FZD2 Antibody - #AF5282

Fig. 5 Adipose tissue-specific omentin overexpression inhibited WNT5A/Ca2+ signaling pathway and improved mitochondrial biogenesis to ameliorate myocardial injury in HF mice. (A) Serum omentin level of HF patients and healthy subjects. A total of 58 HF patients and 38 healthy subjects were enrolled. (B) The omentin serum content of adipose tissue-specific omentin overexpression by intravenous injection of AAV-omentin and negative control by intravenous injection of AAV-NC (n = 8). (C) The expression of omentin in the inguinal fat of mice with adipose tissue-specific omentin overexpression was detected by immunohistochemistry (n = 4). Scale bar = 200 μm. (D) Representative echocardiographs of mice with AAV-omentin overexpression and the statistical results of (E) LV EF, (F) LV FS, (G) stroke volume, (H) LVPW; d, (I) IVS; d, (J) RWT and (K) LV Mass were presented (n = 8). (L) Representative TTC staining images of heart tissues of mice with AAV-omentin overexpression (n = 6). (M) Representative images of H&E and Masson staining of heart tissues of mice with AAV-omentin overexpression (n = 3), scale bar = 250 μm. (N) The serum content of BNP in mice with AAV-omentin overexpression (n = 8). (O) The serum CK activity in mice with AVV-omentin overexpression (n = 8). (P) The protein expression level of WNT5A, Frizzled2 and p-CAMKII/CAMKII were determined by Western blot. β-actin was used as a loading control (n = 5). (Q) The protein expression level of PGC-1α, NRF1, NRF2 and TFAM were determined by Western blot in mice with AAV-omentin overexpression. β-actin was used as a loading control (n = 5). Values are expressed as the means ± SD. (A) Unpaired Student's two-tailed t-test; (B–Q) One-way ANOVA followed by the Dunnett's post hoc test. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Sham; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CAL.

FIGURE 10 Effects of 10- Gin on the Wnt5a (a, c, e) and Frizzled- 2 (b, d, f) gene and protein expressions. Data are represented as mean ± SEM. ** p <.01 versus. CONT; # p <.05, ## p <.01 versus. H/R group, n = 3