GRP94 Antibody - #AF5287

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产品: GRP94 抗体
货号: AF5287
描述: Rabbit polyclonal antibody to GRP94
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P14625
RRID: AB_2837773

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GRP94 Antibody detects endogenous levels of total GRP94.
RRID:
AB_2837773
引用格式: Affinity Biosciences Cat# AF5287, RRID:AB_2837773.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

抗原和靶标

免疫原:

A synthesized peptide derived from human GRP94, corresponding to a region within C-terminal amino acids.

基因/基因ID:
HSP90B1(7184)
描述:
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

研究领域

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

文献引用

1). GRK2 mediates cisplatin-induced acute liver injury via the modulation of NOX4. Cell biology and toxicology, 2024 (PubMed: 39546067) [IF=5.3]
2). Structure‒function‒pathogenicity analysis of C-terminal myocilin missense variants based on experiments and 3D models. Frontiers in Genetics, 2022 (PubMed: 36267417) [IF=3.7]

Application: IF/ICC    Species: Human    Sample:

FIGURE 4 Nonsecreted MYOC mutants induce ER stress and impair autophagy. (A,B) Confocal double immunofluorescence for myocilin (red) and Grp94 (green) in iHTMCs transiently expressing WT or mutated MYOC. Nonsecreted mutations, including S425P and D380Y, show considerable colocalization of myocilin with Grp94. Scale bar: 25 μm. (C,D) iHTMCs expressing WT or mutant myocilins were immunolabeled with LC3 (green) and MYOC (red). S425P and D380Y mutants present increased intracellular myocilin and LC3, with no colocalization of these two proteins. Scale bar: 25 μm.
3). MYOC/p.G367R mutation induces cell dysfunction of the trabecular meshwork and retina via impairment of the protein degradation mechanism. Research Square, 2022

Application: WB    Species: human    Sample: TM cells

Figure 3. G367R-myocilin induced chronic ER stress in TM cells. (A-B) Confocal double immunofluorescence for myocilin (red) and Grp94 (green) in pHTMCs (A) or iHTMCs (B) transiently expressing WT- or G367R-myocilin. The G367R mutant showed considerable colocalization of myocilin with Grp94. Scale bar: 25 μm. (C-F) iHTMCs stably expressing WT or the G367R mutant of myocilin exhibited increased mRNA expression of GRP78, GRP94 and CHOP (C), as well as increased

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