GRP94 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
IF/ICC
Cites

1/5

Western blot analysis of extracts from HepG2, using GRP94 Antibody.

AF5287 at 1/100 staining Human colorectal cancer by IHC-P.

AF5287 at 1/100 staining Mouse lung tissue by IHC-P.

AF5287 staining HepG2 by IF/ICC.

FIGURE 4 Nonsecreted MYOC mutants induce ER stress and impair autophagy.

产品:	GRP94 抗体
货号:	AF5287
描述:	Rabbit polyclonal antibody to GRP94
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	92 kDa; 92kD(Calculated).
蛋白号:	P14625
RRID:	AB_2837773

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(88%), Dog(82%)

克隆:

Polyclonal

特异性:

GRP94 Antibody detects endogenous levels of total GRP94.

RRID:

AB_2837773
引用格式: Affinity Biosciences Cat# AF5287, RRID:AB_2837773.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

抗原和靶标

免疫原:

Uniprot:

P14625(ENPL_HUMAN) >>Visit HPA database.

基因/基因ID:

HSP90B1(7184)

描述:

Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

序列:

P14625 ENPL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Rabbit

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14625 作为底物

P14625

Site	PTM Type	Source
S42	Phosphorylation	Uniprot
T44	Phosphorylation	Uniprot
N62	N-Glycosylation	Uniprot
S64	Phosphorylation	Uniprot
K72	Ubiquitination	Uniprot
K75	Acetylation	Uniprot
K75	Ubiquitination	Uniprot
S92	Phosphorylation	Uniprot
Y94	Phosphorylation	Uniprot
K95	Ubiquitination	Uniprot
K97	Acetylation	Uniprot
K97	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
N107	N-Glycosylation	Uniprot
S109	Phosphorylation	Uniprot
K114	Acetylation	Uniprot
K114	Ubiquitination	Uniprot
S127	Phosphorylation	Uniprot
T133	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
K140	Acetylation	Uniprot
K142	Ubiquitination	Uniprot
T148	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
K161	Ubiquitination	Uniprot
T165	Phosphorylation	Uniprot
K168	Ubiquitination	Uniprot
S169	Phosphorylation	Uniprot
T171	Phosphorylation	Uniprot
S172	Phosphorylation	Uniprot
K177	Methylation	Uniprot
T179	Phosphorylation	Uniprot
S187	Phosphorylation	Uniprot
Y200	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
N217	N-Glycosylation	Uniprot
S225	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
S256	Phosphorylation	Uniprot
Y258	Phosphorylation	Uniprot
K265	Ubiquitination	Uniprot
K270	Ubiquitination	Uniprot
Y280	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
K340	Ubiquitination	Uniprot
S347	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
K360	Ubiquitination	Uniprot
S385	Phosphorylation	Uniprot
T391	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
R395	Methylation	Uniprot
Y401	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
K404	Acetylation	Uniprot
K404	Ubiquitination	Uniprot
K405	Ubiquitination	Uniprot
Y408	Phosphorylation	Uniprot
K410	Ubiquitination	Uniprot
Y429	Phosphorylation	Uniprot
K434	Ubiquitination	Uniprot
S439	Phosphorylation	Uniprot
N445	N-Glycosylation	Uniprot
S447	Phosphorylation	Uniprot
K455	Ubiquitination	Uniprot
K458	Ubiquitination	Uniprot
K467	Ubiquitination	Uniprot
T468	Phosphorylation	Uniprot
K473	Ubiquitination	Uniprot
K479	Acetylation	Uniprot
K479	Ubiquitination	Uniprot
K486	Methylation	Uniprot
K486	Ubiquitination	Uniprot
K493	Ubiquitination	Uniprot
S501	Phosphorylation	Uniprot
T504	Phosphorylation	Uniprot
R511	Methylation	Uniprot
S515	Phosphorylation	Uniprot
T519	Phosphorylation	Uniprot
Y527	Phosphorylation	Uniprot
S544	Phosphorylation	Uniprot
S545	Phosphorylation	Uniprot
S551	Phosphorylation	Uniprot
S552	Phosphorylation	Uniprot
K561	Ubiquitination	Uniprot
K586	Acetylation	Uniprot
K586	Sumoylation	Uniprot
K586	Ubiquitination	Uniprot
K593	Ubiquitination	Uniprot
T604	Phosphorylation	Uniprot
K613	Acetylation	Uniprot
K613	Ubiquitination	Uniprot
K623	Ubiquitination	Uniprot
K633	Ubiquitination	Uniprot
T641	Phosphorylation	Uniprot
S643	Phosphorylation	Uniprot
Y652	Phosphorylation	Uniprot
K663	Methylation	Uniprot
K663	Ubiquitination	Uniprot
Y667	Phosphorylation	Uniprot
T669	Phosphorylation	Uniprot
K671	Ubiquitination	Uniprot
S674	Phosphorylation	Uniprot
T675	Phosphorylation	Uniprot
Y677	Phosphorylation	Uniprot
Y678	Phosphorylation	Uniprot
S680	Phosphorylation	Uniprot
K682	Acetylation	Uniprot
K682	Ubiquitination	Uniprot
K683	Ubiquitination	Uniprot
T684	Phosphorylation	Uniprot
R690	Methylation	Uniprot
Y727	Phosphorylation	Uniprot
K733	Ubiquitination	Uniprot
Y735	Phosphorylation	Uniprot
R738	Methylation	Uniprot
R744	Methylation	Uniprot
S746	Phosphorylation	Uniprot
T774	Phosphorylation	Uniprot
T786	Phosphorylation	Uniprot
T792	Phosphorylation	Uniprot

研究背景

P14625_ENPL_HUMAN

功能:

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).

细胞定位:

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

亚基结构:

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with METTL23.

蛋白家族:

Belongs to the heat shock protein 90 family.

研究领域

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

文献引用

1). Structure‒function‒pathogenicity analysis of C-terminal myocilin missense variants based on experiments and 3D models. Frontiers in Genetics, 2022 (PubMed: 36267417) [IF=3.7]

Application: IF/ICC Species: Human Sample:

FIGURE 4 Nonsecreted MYOC mutants induce ER stress and impair autophagy. (A,B) Confocal double immunofluorescence for myocilin (red) and Grp94 (green) in iHTMCs transiently expressing WT or mutated MYOC. Nonsecreted mutations, including S425P and D380Y, show considerable colocalization of myocilin with Grp94. Scale bar: 25 μm. (C,D) iHTMCs expressing WT or mutant myocilins were immunolabeled with LC3 (green) and MYOC (red). S425P and D380Y mutants present increased intracellular myocilin and LC3, with no colocalization of these two proteins. Scale bar: 25 μm.

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GRP94 Antibody - #AF5287

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