(1)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5289, RRID:AB_2837774.
展开/折叠
ATP sensitive inward rectifier potassium channel 11; Beta cell inward rectifier subunit; BIR; HHF 2; HHF2; IKATP; Inward rectifier K(+) channel Kir6.2; Inwardly rectifying potassium channel KIR6.2; IRK 11; IRK11; KCNJ11; Kir 6.2; Kir6.2; MGC133230; PHHI; Potassium channel inwardly rectifing subfamily J member 11; Potassium channel, inwardly rectifying subfamily J member 11; Potassium inwardly rectifying channel J11; TNDM 3; TNDM3;
抗原和靶标
- Q14654 KCJ11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSRKGIIPEEYVLTRLAEDPAEPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14654 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T180 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S208 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Q9UQM7 (CAMK2A) , Q13557 (CAMK2D) | Uniprot |
| K332 | Ubiquitination | Uniprot | |
| T341 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | P28482 (MAPK1) | Uniprot |
研究背景
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.
Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.
Membrane>Multi-pass membrane protein.
Interacts with ABCC8/SUR. Interacts with ABCC9/SUR2.
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
文献引用
Application: IF/ICC Species: Human Sample: WERI-Rb1 cells
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