产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5295, RRID:AB_2837780.
展开/折叠
6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT; MGMT_HUMAN; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase;
抗原和靶标
- P16455 MGMT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN
翻译修饰 - P16455 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| S201 | Phosphorylation | Uniprot |
研究背景
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Nucleus.
Belongs to the MGMT family.
限制条款
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