AGER/RAGE Antibody - #AF5309

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产品: AGER/RAGE 抗体
货号: AF5309
描述: Rabbit polyclonal antibody to AGER/RAGE
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 43~60kDa; 43kD(Calculated).
蛋白号: Q15109
RRID: AB_2837794

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(100%), Dog(83%)
克隆:
Polyclonal
特异性:
AGER/RAGE Antibody detects endogenous levels of total AGER/RAGE.
RRID:
AB_2837794
引用格式: Affinity Biosciences Cat# AF5309, RRID:AB_2837794.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Advanced glycosylation end product-specific receptor; Ager; MGC2235; RAGE_HUMAN; Receptor for advanced glycosylation end products;

抗原和靶标

免疫原:
Uniprot:

Q15109(RAGE_HUMAN) >>Visit HPA database.

基因/基因ID:
AGER(177)
表达:
Q15109 RAGE_HUMAN:

Endothelial cells.

描述:
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes.
序列:
MAAGTAVGAWVLVLSLWGAVVGAQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALALGILGGLGTAALLIGVILWQRRQRRGEERKAPENQEEEEERAELNQSEEPEAGESSTGGP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
92
Horse
83
Bovine
83
Sheep
83
Dog
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15109 作为底物

Site PTM Type Enzyme
S391 Phosphorylation
S399 Phosphorylation
S400 Phosphorylation

研究背景

功能:

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Endothelial cells.

亚基结构:

Interacts with S100A1 and APP (By similarity). Interacts with S100B, S100A12 and S100A14. Constitutive homodimer; disulfide-linked.

文献引用

1). Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis. Cell Death Discovery, 2022 (PubMed: 35091537) [IF=7.0]

Application: IF/ICC    Species: Human    Sample: lung tissues

Fig. 4: Single-Cell transcriptional analysis unravels heterogeneity of fibroblasts during FHP.A t-SNE visualization of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts. B The heatmap showing the expression levels of top five marker genes in fibroblast subpopulations. C Relative percentage of fibroblast subpopulations in lung from the patient with FHP. D Violin plots displaying the expression levels of the representative genes in each fibroblast subpopulation. E Relative gene set enrichment scores of subpopulations calculated by AUCell analysis. F Multiplex immunofluorescence images of TCF21, ACTA2, and COL1A1 in lung tissues from FHP. Scale bar = 10 μm. G Trajectory analysis of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts using Monocle 2 (upper) and Velocyto R package (lower). H CEBPD_extended motif showed greater enrichment of regulon activity for PLA2G2Ahigh fibroblasts using SCENIC analysis (left panel). MEF2C_extended motif showed greater enrichment of regulon activity for ACTA2high fibroblasts (left panel). t-SNE visualization of AUC values of CEBPD_extended and MEF2C_extended motifs in fibroblasts (right panel). I Schematic developmental trajectories of fibroblast subpopulations in FHP lungs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
2). Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis. LIFE SCIENCES, 2020 (PubMed: 32702445) [IF=6.1]

Application: IF/ICC    Species: rat    Sample: HepG2 cells

Fig. 4. |Naringin ameliorates NASH through modulation of the AGE/RAGE mechanism. The level of AGE was determined in HepG2 cells and plasma by (A) ELISA and(B) fluorimetry. Receptor for AGE (RAGE) was analyzed in HepG2 cells and liver tissue by (C) qPCR (n = 6) and (D and E) immunoblotting (n = 3). Expressions were normalized by GAPDH. RAGE expression was further validated by immunofluorescence in (F) cells, (G) its quantification (n = 5), and (H) tissue and (I) its quantification (n = 5) by using Alexa Fluor 594 (red), DAPI (blue). Magnification 40×. Data are shown as Mean ± S.E.M (n = 8), *Control vs NASH; #NASH vs NAR. **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001.

Application: WB    Species: rat    Sample: HepG2 cells and liver tissue

Fig. 4. |Naringin ameliorates NASH through modulation of the AGE/RAGE mechanism. The level of AGE was determined in HepG2 cells and plasma by (A) ELISA and(B) fluorimetry. Receptor for AGE (RAGE) was analyzed in HepG2 cells and liver tissue by (C) qPCR (n = 6) and (D and E) immunoblotting (n = 3). Expressions were normalized by GAPDH. RAGE expression was further validated by immunofluorescence in (F) cells, (G) its quantification (n = 5), and (H) tissue and (I) its quantification (n = 5) by using Alexa Fluor 594 (red), DAPI (blue). Magnification 40×. Data are shown as Mean ± S.E.M (n = 8), *Control vs NASH; #NASH vs NAR. **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001.
3). Identification of S100A8/A9 involved in thromboangiitis obliterans development using tandem mass tags-labeled quantitative proteomics analysis. Cellular signalling, 2024 (PubMed: 38697446) [IF=4.3]
4). Network-pharmacology-based research on protective effects and underlying mechanism of Shuxin decoction against myocardial ischemia/reperfusion injury with diabetes. World journal of diabetes, 2023 (PubMed: 37547579) [IF=4.2]
5). HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma. OncoTargets and Therapy, 2018 (PubMed: 30122942) [IF=4.0]

Application: WB    Species: human    Sample: RCC cell

Figure 1 |HMGB1 knockdown decreased the viability of A498 and ACHN cells.Notes: (A) The RAGE and HMGB1 proteins in RCC cell lines were detected by Western blot.
6). Anti-high-mobility group box-1 (HMGB1) mediates the apoptosis of alveolar epithelial cells (AEC) by receptor of advanced glycation end-products (RAGE)/c-Jun N-terminal kinase (JNK) pathway in the rats of crush injuries. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35033142) [IF=2.6]

Application: WB    Species: Rat    Sample: lung tissue

Fig. 2 EP, FPS-ZM1, and SP600125 affect the expression of HMGB1, RAGE, and JNK in lung tissue. Relative levels of HMGB1, RAGE, and JNK were analyzed using Western blot. The HMGB1, RAGE, and p-JNK were lower in the later three pretreatment groups. Results were expressed as means ± SDs. The number of rats were 13, 12, 14, 13, and 14 in CS, CS + vehicle, CS + EP, CS + FPS-ZM1, and CS + SP600125 groups, respectively. Statistical significance: ns P ≥ 0.05, *P < 0.05 and **P < 0.01, versus CS group

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