Wnt1 Antibody - #AF5315

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产品: Wnt1 抗体
货号: AF5315
描述: Rabbit polyclonal antibody to Wnt1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 41 kDa; 41kD(Calculated).
蛋白号: P04628
RRID: AB_2837800

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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
克隆:
Polyclonal
特异性:
Wnt1 Antibody detects endogenous levels of total Wnt1.
RRID:
AB_2837800
引用格式: Affinity Biosciences Cat# AF5315, RRID:AB_2837800.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BMND16; INT1; OI15; oncogene Int1; Proto oncogene protein Wnt 1; Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; Wingless type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1); Wnt 1; wnt1; WNT1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P04628(WNT1_HUMAN) >>Visit HPA database.

基因/基因ID:
WNT1(7471)
描述:
Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
序列:
MGLWALLPGWVSATLLLALAALPAALAANSSGRWWGIVNVASSTNLLTDSKSLQLVLEPSLQLLSRKQRRLIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTAPGPHLFGKIVNRGCRETAFIFAITSAGVTHSVARSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTVFSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPAHKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCNCTFHWCCHVSCRNCTHTRVLHECL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Zebrafish
55
Xenopus
45
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P04628 作为底物

Site PTM Type Enzyme
S132 Phosphorylation
T136 Phosphorylation
S144 Phosphorylation
T348 Phosphorylation
S356 Phosphorylation

研究背景

功能:

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis.

翻译修饰:

Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).

细胞定位:

Secreted>Extracellular space>Extracellular matrix. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids. Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3 (By similarity). Interacts with WLS (By similarity).

蛋白家族:

Belongs to the Wnt family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

文献引用

1). XQ-1H alleviates cerebral ischemia in mice through inhibition of apoptosis and promotion of neurogenesis in a Wnt/β-catenin signaling dependent way. LIFE SCIENCES, 2019 (PubMed: 31499069) [IF=6.1]
2). Yanghe Decoction Suppresses the Experimental Autoimmune Thyroiditis in Rats by Improving NLRP3 Inflammasome and Immune Dysregulation. Frontiers in Pharmacology, 2021 (PubMed: 34234669) [IF=5.6]

Application: WB    Species: Rat    Sample: peripheral blood mononuclear cell (PBMCs)

FIGURE 7 RT-PCR and Western blot analysis for the expression of molecules in the Wnt/β-catenin signaling pathway in peripheral blood mononuclear cell (PBMCs) (A) mRNA expression of Wnt-1 and β-catenin (B) Western blotting images of Wnt-1and β-catenin: Lane 1, control group; lane 2, model group; lane 3, YH 5 g crude drug/kG group; lane 4, YH 15 g crude drug/kG group; lane 5, selenious group (C) Bar graphs indicate the relative ratio of Wnt-1and β-catenin over tubulin. All values are expressed as mean ± SEM. *p < 0.05, **p < 0.01 vs Model.
3). Phillygenin Inhibits TGF-β1-induced Hepatic Stellate Cell Activation and Inflammation: Regulation of the Bax/Bcl-2 and Wnt/β-catenin Pathways. Inflammation, 2024 (PubMed: 38393550) [IF=5.1]

Application: WB    Species: Mouse    Sample: mHSC

Fig. 10 Efects of PHI on Wnt/β-catenin signaling pathway-related proteins. a Western blotting analyses of Wnt1, β-catenin, GSK-3β, p-GSK-3β, c-Myc, and Cyclin D1 in mHSCs.
4). Absence of the Klotho Function Causes Cornea Degeneration with Specific Features Resembling Fuchs Endothelial Corneal Dystrophy and Bullous Keratopathy. Biology, 2024 [IF=4.2]

Application: IHC    Species: Human    Sample:

Figure 6. Representative photographs of corneal immunohistochemical detection with Wnt1 antibody and quantification analysis. (A) Wnt1 immunodetection in a male cornea. (B) Wnt1 immunodetection in a female cornea. (C–E) For the quantitative analysis, the Wnt1 expression is presented as a Wnt1-positive area/central cornea area, peripheral cornea area, and limbus area in Figures (C–E), respectively. Wnt-1 expression increases with the Klotho null mutation in both genders, although without statistical significance (C,D). In the limbus, Wnt-1 expression also increases in the male Klotho null mutants. In the limbus of the female mutants, a significant decrease in Wnt-1 expression can be seen when compared with the Klotho heterozygous mutants (E). * p < 0.05. Scale bars represent 25 μm.
5). Indoxyl sulfate promotes the atherosclerosis through up-regulating the miR-34a expression in endothelial cells and vascular smooth muscle cells in vitro. VASCULAR PHARMACOLOGY, 2020 (PubMed: 32593718) [IF=4.0]

Application: WB    Species: human    Sample: HUVECs and HA-VSMCs

Fig. 7.| IS inhibits the Notch signaling pathway and miR-34a-related protein. After transfection with miR-34a mimics (50 nM) or inhibitor (100 nM), IS (1000 μM) was added and incubated for another 36 h. Western blotting was used to detected the protein expression in HUVECs and HA-VSMCs. Data are represented as the mean ± SEM (n = 3). ⁎P < .05, versus control, #P < .05, versus IS group by one-way ANOVA with Tukey's test
6). Adipose derived mesenchymal stem cells alleviated osteoarthritis and chondrocyte apoptosis through autophagy inducing. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 30315711) [IF=4.0]
7). Tangshen Formula Improves Diabetes-Associated Myocardial Fibrosis by Inhibiting TGF-β/Smads and Wnt/β-Catenin Pathways. Frontiers in Medicine, 2021 (PubMed: 34938743) [IF=3.9]

Application: WB    Species: Mouse    Sample:

Figure 4 An effect of TSF on the canonical Wnt pathway. (A) IHC of anti-β-catenin (original magnification, 200×). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 TSF-treated group vs. KKAy group; #p < 0.05, ##p < 0.01, ###p < 0.001 KKAy group vs. C57BL/6J group. (B) Western blot analyses and semi-quantitative analyses revealed expressions of Wnt1, active-β-catenin, β-catenin, FN, and MMP-7 in KKAy mice and the different treatment groups. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 KKAy group vs. C57BL/6J group. *p < 0.05, **p < 0.01 TSF-treated group vs. KKAy group.
8). Chemokine (C-C motif) ligand 2-enhanced adipogenesis and angiogenesis of human adipose-derived stem cell and human umbilical vein endothelial cell co-culture system in adipose tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 2022 (PubMed: 34811942) [IF=3.3]
9). Neoisoliquiritigenin Inhibits Tumor Progression by Targeting GRP78-β- catenin Signaling in Breast Cancer. CURRENT CANCER DRUG TARGETS, 2018 (PubMed: 28914191) [IF=3.0]
10). Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells. Experimental and Therapeutic Medicine, 2020 (PubMed: 32256772) [IF=2.7]

Application: WB    Species: Human    Sample: SW1353 cells

Figure 4 Effect of irisin on the activation of the Wnt/β-catenin signaling pathway in IL-1β-induced SW1353 cells. SW1353 cells were treated with 20 mM irisin for 24 h, with or without IL-1β, and (A) β-catenin protein levels, (B) Wnt-1 protein levels and (C) β-catenin and Wnt-1 protein expression,were evaluated by western blot analysis. n=5/group. mRNA expression levels of (D) β-catenin and (E) Wnt-1 were evaluated by reverse transcription-quantitative PCR. n=5/group. *P<0.05, **P<0.01 and ***P<0.001. IL-1β, interleukin-1β; NC, negative control.
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