产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5317, RRID:AB_2837802.
展开/折叠
AT like disease; Ataxia telangiectasia disorder like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; Meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A (S. cerevisiae); Meiotic recombination 11 homolog A; MmMRE11A; Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A; MRE11_HUMAN; MRE11A; MRE11b; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833;
抗原和靶标
- P49959 MRE11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEEINFGKLITKPSEGTTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERHIDALEDKIDEEVRRFRETRQKNTNEEDDEVREAMTRARALRSQSEESASAFSADDLMSIDLAEQMANDSDDSISAATNKGRGRGRGRRGGRGQNSASRGGSQRGRADTGLETSTRSRNSKTAVSASRNMSIIDAFKSTRQQPSRNVTTKNYSEVIEVDESDVEEDIFPTTSKTDQRWSSTSSSKIMSQSQVSKGVDFESSEDDDDDPFMNTSSLRRNRR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49959 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| S264 | Phosphorylation | Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| T272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| S382 | Phosphorylation | Q13535 (ATR) | Uniprot |
| K384 | Ubiquitination | Uniprot | |
| R388 | Methylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K416 | Acetylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| T426 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| K480 | Acetylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| T481 | Phosphorylation | Uniprot | |
| K486 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| S531 | Phosphorylation | Q13535 (ATR) | Uniprot |
| S558 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| R570 | Methylation | Uniprot | |
| R572 | Methylation | Uniprot | |
| R574 | Methylation | Uniprot | |
| R576 | Methylation | Uniprot | |
| R577 | Methylation | Uniprot | |
| R580 | Methylation | Uniprot | |
| R587 | Methylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| R592 | Methylation | Uniprot | |
| R594 | Methylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| R604 | Methylation | Uniprot | |
| S613 | Phosphorylation | Uniprot | |
| R616 | Methylation | Uniprot | |
| S619 | Phosphorylation | Uniprot | |
| K625 | Sumoylation | Uniprot | |
| S649 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S667 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| S676 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S678 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S681 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S689 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot |
研究背景
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.
Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.
Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity. Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain).
(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.
Belongs to the MRE11/RAD32 family.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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