产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5319, RRID:AB_2837804.
展开/折叠
Gcgr; GGR; GL R; GL-R; GLR; GLR_HUMAN; Glucagon receptor; Glucagon receptor precursor; MGC138246;
抗原和靶标
- P47871 GLR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPPCQPQRPLLLLLLLLACQPQVPSAQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAILGGLSKLHCTRNAIHANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVAGCRVAAVFMQYGIVANYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVPWAVVKCLFENVQCWTSNDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHHTDYKFRLAKSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVLYCFLNKEVQSELRRRWHRWRLGKVLWEERNTSNHRASSSPGHGPPSKELQFGRGGGSQDSSAETPLAGGLPRLAESPF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P47871 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| N46 | N-Glycosylation | Uniprot | |
| N59 | N-Glycosylation | Uniprot | |
| N74 | N-Glycosylation | Uniprot | |
| N78 | N-Glycosylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot |
研究背景
G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding.
Cell membrane>Multi-pass membrane protein.
Note: Is rapidly internalized after ligand-binding.
Belongs to the G-protein coupled receptor 2 family.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
限制条款
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