产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5323, RRID:AB_2837808.
展开/折叠
Activating enhancer binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP 2 transcription factor; AP 2alpha; AP-2; AP-2 transcription factor; AP2; AP2 Transcription Factor; AP2-alpha; AP2A_HUMAN; AP2TF; BOFS; FLJ51761; TFAP 2; TFAP 2A; TFAP2; TFAP2A; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha; Transcription factor AP2 alpha;
抗原和靶标
- P05549 AP2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLWKLTDNIKYEDCEDRHDGTSNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHALSSGLGDLSIHSLPHAIEEVPHVEDPGINIPDQTVIKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVTRKNMLLATKQICKEFTDLLAQDRSPLGNSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNNAKSSDKEEKHRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05549 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K10 | Sumoylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K177 | Sumoylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S258 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K268 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| K335 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Sumoylated on Lys-10; which inhibits transcriptional activity.
Nucleus.
Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Interacts with KCTD15; this interaction inhibits TFAP2A transcriptional activation.
The PPxY motif mediates interaction with WWOX.
Belongs to the AP-2 family.
限制条款
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