PIAS4 Antibody - #AF5329

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产品: PIAS4 抗体
货号: AF5329
描述: Rabbit polyclonal antibody to PIAS4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Dog, Chicken
分子量: 57 kDa; 57kD(Calculated).
蛋白号: Q8N2W9
RRID: AB_2837814

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
PIAS4 Antibody detects endogenous levels of total PIAS4.
RRID:
AB_2837814
引用格式: Affinity Biosciences Cat# AF5329, RRID:AB_2837814.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; Pias4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6;

抗原和靶标

免疫原:
Uniprot:

Q8N2W9(PIAS4_HUMAN) >>Visit HPA database.

基因/基因ID:
PIAS4(51588)
表达:
Q8N2W9 PIAS4_HUMAN:

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

描述:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway.
序列:
MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
83
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8N2W9 作为底物

Site PTM Type Enzyme
A2 Acetylation
K9 Sumoylation
K9 Ubiquitination
S18 Phosphorylation
K31 Ubiquitination
K35 Sumoylation
K35 Ubiquitination
K56 Ubiquitination
K59 Sumoylation
K59 Ubiquitination
K68 Ubiquitination
K69 Sumoylation
K69 Ubiquitination
T99 Phosphorylation
Y108 Phosphorylation
K114 Acetylation
K114 Ubiquitination
K125 Acetylation
K125 Ubiquitination
K128 Sumoylation
K128 Ubiquitination
K135 Sumoylation
K158 Ubiquitination
K187 Ubiquitination
K231 Sumoylation
K231 Ubiquitination
Y274 Phosphorylation
K294 Ubiquitination
K300 Ubiquitination
K306 Sumoylation
K306 Ubiquitination
K330 Ubiquitination
K360 Ubiquitination
K411 Ubiquitination
K504 Ubiquitination

翻译修饰 - Q8N2W9 作为激酶

Substrate Site Source
P15927 (RPA2) S4 Uniprot
P15927 (RPA2) S8 Uniprot

研究背景

功能:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.

翻译修饰:

Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.

细胞定位:

Nucleus>PML body.
Note: Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.

亚基结构:

Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with MTA1.

蛋白家族:

The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.

Belongs to the PIAS family.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

文献引用

1). Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose. Molecular Medicine Reports, 2023 (PubMed: 36601740) [IF=3.4]

Application: WB    Species: Human    Sample: HK-2 cells

Figure 4. High glucose upregulates STAT1 SUMOylation in HK-2 cells. (A) Co-IP suggested that the binding of STAT1 and UBC9 was upregulated under high-glucose conditions. The data were analyzed by Student's t-test. (B) Efficiency of UBC9 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and Tukey post hoc test. (C and D) Co-IP suggested that the SUMOylation of STAT1 was enhanced under high-glucose conditions. The data were analyzed by one-way ANOVA and Tukey post hoc test. (E) Binding of STAT1 and PIASs was analyzed. Results suggested that PIAS4 mediated the upregulation of STAT1 SUMOylation. The data were analyzed by Student's t-test. (F) Efficiency of PIAS4 knockdown was analyzed by western blotting and was semi-quantified using densitometry. The data were analyzed by one-way ANOVA and LSD post hoc test. The effects of PIAS4 siRNA transfection on STAT1 SUMOylation (G) were analyzed by co-IP and (H) were semi-quantified using densitometry. The results suggested that PIAS4 knockdown inhibited STAT1 SUMOylation. The data were analyzed by one-way ANOVA and Tukey post hoc test. IP:STAT1 means the cell lysate was incubated with STAT1 antibody. Input was used as a positive control to detect the presence of target proteins. n=3. *P

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