FRA2 Antibody - #AF5345

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产品: FRA2 抗体
货号: AF5345
描述: Rabbit polyclonal antibody to FRA2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 35 kDa; 35kD(Calculated).
蛋白号: P15408
RRID: AB_2837830

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
FRA2 Antibody detects endogenous levels of total FRA2.
RRID:
AB_2837830
引用格式: Affinity Biosciences Cat# AF5345, RRID:AB_2837830.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; Fos-related antigen 2; FosL 2; Fosl2; FOSL2_HUMAN; FRA 2; FRA-2;

抗原和靶标

免疫原:
Uniprot:

P15408(FOSL2_HUMAN) >>Visit HPA database.

基因/基因ID:
FOSL2(2355)
描述:
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts.
序列:
MYQDYPGNFDTSSRGSSGSPAHAESYSSGGGGQQKFRVDMPGSGSAFIPTINAITTSQDLQWMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKRRIRRERNKLAAAKCRNRRRELTEKLQAETEELEEEKSGLQKEIAELQKEKEKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P15408 作为底物

Site PTM Type Enzyme
M1 Acetylation
Y2 Phosphorylation
Y5 Phosphorylation
S16 Phosphorylation
S17 Phosphorylation
S19 Phosphorylation
K35 Ubiquitination
S79 Phosphorylation
S83 Phosphorylation
K104 Acetylation
K104 Methylation
K104 Ubiquitination
S120 Phosphorylation
K151 Ubiquitination
K168 Ubiquitination
S194 Phosphorylation
S200 Phosphorylation
S211 Phosphorylation
S215 Phosphorylation
K222 Acetylation
K222 Sumoylation
S230 Phosphorylation
S232 Phosphorylation
S233 Phosphorylation
S235 Phosphorylation
K240 Acetylation
K240 Ubiquitination
S307 Phosphorylation
S308 Phosphorylation
S309 Phosphorylation
S310 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
T322 Phosphorylation

研究背景

功能:

Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer.

蛋白家族:

Belongs to the bZIP family. Fos subfamily.

研究领域

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). FOSL2 participates in renal fibrosis via SGK1-mediated epithelial-mesenchymal transition of proximal tubular epithelial cells. Journal of Translational Internal Medicine, 2023 (PubMed: 37662889) [IF=4.9]

Application: WB    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.

Application: IHC    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.

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