产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5351, RRID:AB_2837836.
展开/折叠
ACT; ACTA3; ACTE; ACTG2; ACTH_HUMAN; Actin; Actin gamma 2 smooth muscle enteric; Actin gamma enteric smooth muscle; Actin like protein; ACTL3; ACTSG; Alpha actin 3; Alpha-actin-3; Gamma 2 actin; Gamma-2-actin; gamma-enteric smooth muscle; Smooth muscle gamma actin; Smooth muscle gamma-actin;
抗原和靶标
- P63267 ACTH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P63267 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| E3 | Acetylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Methylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Sumoylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| K69 | Methylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Methylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| R96 | Methylation | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Methylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| K316 | Acetylation | Uniprot | |
| K316 | Sumoylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K327 | Methylation | Uniprot | |
| K327 | Sumoylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K329 | Acetylation | Uniprot | |
| K329 | Sumoylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K337 | Sumoylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot |
研究背景
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-74 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Cytoplasm>Cytoskeleton.
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Belongs to the actin family.
研究领域
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
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