产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5358, RRID:AB_2837843.
展开/折叠
Octamer binding transcription factor 4; MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1_HUMAN; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1;
抗原和靶标
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
- Q01860 PO5F1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q01860 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| T116 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K123 | Sumoylation | Uniprot | |
| K140 | Methylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K144 | Methylation | Uniprot | |
| K151 | Acetylation | Uniprot | |
| K151 | Methylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K154 | Acetylation | Uniprot | |
| K154 | Methylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K156 | Acetylation | Uniprot | |
| K156 | Methylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| K177 | Methylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| R186 | Methylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Methylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Methylation | Uniprot | |
| K206 | Methylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | P31751 (AKT2) , P31749 (AKT1) , Q9Y243 (AKT3) | Uniprot |
| S236 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | P11309 (PIM1) , P17612 (PRKACA) | Uniprot |
| S289 | Phosphorylation | P11309 (PIM1) , P17612 (PRKACA) | Uniprot |
| S290 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S359 | Phosphorylation | Uniprot |
研究背景
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Cytoplasm. Nucleus.
Note: Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Interacts with PKM. Interacts with WWP2. Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PCGF1. Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity). Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity). Interacts with FBXW4 (By similarity). Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity).
The POU-specific domain mediates interaction with PKM.
Belongs to the POU transcription factor family. Class-5 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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