产品: Hsp40 抗体
货号: AF5363
描述: Rabbit polyclonal antibody to Hsp40
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 38 kDa; 38kD(Calculated).
蛋白号: P25685
RRID: AB_2837848

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Hsp40 Antibody detects endogenous levels of total Hsp40.
RRID:
AB_2837848
引用格式: Affinity Biosciences Cat# AF5363, RRID:AB_2837848.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DnaJ (Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
序列:
MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
91
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P25685 作为底物

Site PTM Type Enzyme
K3 Ubiquitination
Y5 Phosphorylation
R13 Methylation
S16 Phosphorylation
K21 Ubiquitination
K35 Ubiquitination
K37 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K46 Ubiquitination
Y52 Phosphorylation
K60 Ubiquitination
R66 Methylation
T88 Phosphorylation
S89 Phosphorylation
S149 Phosphorylation Q8IW41 (MAPKAPK5)
S151 Phosphorylation Q8IW41 (MAPKAPK5)
K159 Ubiquitination
S171 Phosphorylation Q8IW41 (MAPKAPK5)
Y176 Phosphorylation
S177 Phosphorylation
T180 Phosphorylation
K181 Acetylation
K181 Ubiquitination
S186 Phosphorylation
K195 Acetylation
K195 Ubiquitination
S196 Phosphorylation
K202 Ubiquitination
T205 Phosphorylation
K217 Ubiquitination
K222 Ubiquitination
T227 Phosphorylation
S228 Phosphorylation
K240 Ubiquitination
K242 Ubiquitination
K248 Ubiquitination
S252 Phosphorylation
Y256 Phosphorylation
S261 Phosphorylation
K286 Ubiquitination
K296 Ubiquitination
K306 Ubiquitination
T307 Phosphorylation

研究背景

功能:

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).

细胞定位:

Cytoplasm. Nucleus. Nucleus>Nucleolus.
Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with DNAJC3. Interacts with HSF1 (via transactivation domain); this interaction results in the inhibition of heat shock- and HSF1-induced transcriptional activity during the attenuation and recovery phase period of the heat shock response.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

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