Cleaved-Caspase 6 (Asp179) Antibody - #AF5381
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5381, RRID:AB_2837866.
展开/折叠
Apoptotic protease Mch-2; Apoptotic protease MCH2; CASP-6; CASP6; CASP6_HUMAN; Caspase 6; Caspase 6 apoptosis related cysteine protease; Caspase 6, apoptosis related cysteine peptidase; Caspase-6 subunit p11; Mch2; OTTHUMP00000162742; OTTHUMP00000162743;
抗原和靶标
- P55212 CASP6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P55212 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R9 | Methylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K36 | Acetylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | O60285 (NUAK1) | Uniprot |
| K265 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K273 | Acetylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K285 | Ubiquitination | Uniprot |
研究背景
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Cytoplasm.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.
Belongs to the peptidase C14A family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
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