产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5390, RRID:AB_2837875.
展开/折叠
C5DELq14.3; DEL5q14.3; MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C); MADS box transcription enhancer factor 2, polypeptide C; MEF2C; MEF2C_HUMAN; Myocyte enhancer factor 2C; Myocyte specific enhancer factor 2C; Myocyte-specific enhancer factor 2C; OTTHUMP00000222409; Similar to MADS box transcription enhancer factor 2 polypeptide C;
抗原和靶标
- Q06413 MEF2C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVETLRKKGLNGCDSPDPDADDSVGHSPESEDKYRKINEDIDLMISRQRLCAVPPPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVSEDVDLLLNQRINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSVTGWQQQHLHNMPPSALSQLGACTSTHLSQSSNLSLPSTQSLNIKSEPVSPPRDRTTTPSRYPQHTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPIGLTRPSPDERESPSVKRMRLSEGWAT
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q06413 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T20 | Phosphorylation | Uniprot | |
K23 | Ubiquitination | Uniprot | |
S59 | Phosphorylation | Uniprot | |
K64 | Ubiquitination | Uniprot | |
K68 | Ubiquitination | Uniprot | |
T80 | Phosphorylation | Q9H1R3 (MYLK2) | Uniprot |
S82 | Phosphorylation | Uniprot | |
T87 | Phosphorylation | Uniprot | |
S98 | Phosphorylation | Uniprot | |
S106 | Phosphorylation | Uniprot | |
S110 | Phosphorylation | Uniprot | |
K116 | Acetylation | Uniprot | |
K119 | Acetylation | Uniprot | |
S181 | Phosphorylation | Uniprot | |
S183 | Phosphorylation | Uniprot | |
S192 | Phosphorylation | P51812 (RPS6KA3) | Uniprot |
S222 | Phosphorylation | Uniprot | |
S228 | Phosphorylation | Uniprot | |
K234 | Acetylation | Uniprot | |
K239 | Acetylation | Uniprot | |
S240 | Phosphorylation | Uniprot | |
K252 | Acetylation | Uniprot | |
K264 | Acetylation | Uniprot | |
T293 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
T300 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
S387 | Phosphorylation | Q13164 (MAPK7) , Q16539 (MAPK14) | Uniprot |
T387 | Phosphorylation | Uniprot | |
K391 | Sumoylation | Uniprot | |
S396 | Phosphorylation | P06493 (CDK1) | Uniprot |
S419 | Phosphorylation | Uniprot | |
S445 | Phosphorylation | Uniprot | |
S453 | Phosphorylation | Uniprot | |
S459 | Phosphorylation | Uniprot | |
S461 | Phosphorylation | Uniprot |
研究背景
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.
Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity).
Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
Nucleus. Cytoplasm>Sarcoplasm.
Expressed in brain and skeletal muscle.
Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with EP300 in differentiating cells; the interaction acetylates MEF2C leading to increased DNA binding and activation (By similarity). Interacts with HDAC7 and CARM1 (By similarity). Interacts with HDAC4 and HDAC9; the interaction with HDACs represses transcriptional activity. Interacts with LPIN1. Interacts with MYOCD. Interacts with AKAP13 (By similarity). Interacts with FOXK1; the interaction inhibits MEF2C transactivation activity (By similarity). Interacts (via N-terminus) with HABP4; this interaction decreases DNA-binding activity of MEF2C in myocardial cells in response to mechanical stress. Interacts with JPH2; interaction specifically takes place with the Junctophilin-2 N-terminal fragment cleavage product of JPH2 (By similarity). Interacts (via MADS box) with SOX18 (By similarity).
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
Belongs to the MEF2 family.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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