产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5406, RRID:AB_2837890.
展开/折叠
6330408H09Rik; AI661354; AV102684; AW538719; CAK; CAK complex subunit; ccnh; CCNH_HUMAN; CDK activating kinase; CDK activating kinase complex subunit; Cyclin dependent kinase activating kinase; cyclin dependent kinase activating kinase complex subunit; Cyclin H; Cyclin-H; CyclinH; MO15 associated protein; MO15-associated protein; p34; p36; p37;
抗原和靶标
- P51946 CCNH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51946 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | P49336 (CDK8) | Uniprot |
| K8 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | P49336 (CDK8) | Uniprot |
| S307 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S322 | Phosphorylation | Uniprot |
研究背景
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Nucleus.
Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.
Belongs to the cyclin family. Cyclin C subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
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