ATF4 Antibody - #AF5416

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(4)   (4)  
产品: ATF4 抗体
货号: AF5416
描述: Rabbit polyclonal antibody to ATF4
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Dog
分子量: 39~49kD; 39kD(Calculated).
蛋白号: P18848
RRID: AB_2837900

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(86%), Bovine(86%), Dog(86%)
克隆:
Polyclonal
特异性:
ATF4 Antibody detects endogenous levels of total ATF4.
RRID:
AB_2837900
引用格式: Affinity Biosciences Cat# AF5416, RRID:AB_2837900.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activating transcription factor 4; ATF 4; ATF4; ATF4 protein; ATF4_HUMAN; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; CREB2; Cyclic AMP dependent transcription factor ATF 4; Cyclic AMP response element binding protein 2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA binding protein TAXREB67; DNA-binding protein TAXREB67; Tax Responsive Enhancer Element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB;

抗原和靶标

免疫原:
Uniprot:

P18848(ATF4_HUMAN) >>Visit HPA database.

基因/基因ID:
ATF4(468)
描述:
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production
序列:
MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
86
Bovine
86
Dog
86
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18848 作为底物

Site PTM Type Enzyme
K45 Sumoylation
K45 Ubiquitination
K53 Sumoylation
K53 Ubiquitination
K55 Ubiquitination
S69 Phosphorylation
K75 Ubiquitination
K88 Ubiquitination
T107 Phosphorylation P07949 (RET)
T114 Phosphorylation P07949 (RET)
T115 Phosphorylation P07949 (RET)
T119 Phosphorylation P07949 (RET)
S215 Phosphorylation
S219 Phosphorylation
S224 Phosphorylation
S245 Phosphorylation P51812 (RPS6KA3)
S248 Phosphorylation
K259 Ubiquitination
K267 Sumoylation
K267 Ubiquitination
K277 Ubiquitination
T293 Phosphorylation
Y295 Phosphorylation
K299 Ubiquitination
K311 Acetylation PR:000007102 (EP300)
K329 Ubiquitination
K335 Ubiquitination
K343 Acetylation
K348 Acetylation

研究背景

功能:

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors.

翻译修饰:

Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4.

Phosphorylated by NEK6 (By similarity). Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1 (By similarity).

Phosphorylated by NEK6.

细胞定位:

Cytoplasm. Cell membrane. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Colocalizes with NEK6 at the centrosome (PubMed:20873783).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds DNA as a homodimer and as a heterodimer. Interacts with CEBPB and binds DNA as a heterodimer with CEBPB. Interacts (via its leucine zipper domain) with GABBR1 and GABBR2 (via their C-termini). Interacts (via its DNA binding domain) with FOXO1 (C-terminal half); the interaction occurs in osteoblasts and regulates glucose homeostasis through suppression of beta-cell proliferation and a decrease in insulin production. Interacts with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with CEP290 (via an N-terminal region). Interacts with NEK6, DAPK2 (isoform 2) and ZIPK/DAPK3. Forms a heterodimer with TXLNG in osteoblasts. Interacts with DDIT3/CHOP. Interacts with ABRAXAS2.

蛋白家族:

The BetaTrCP degron motif promotes binding to BTRC when phosphorylated.

Belongs to the bZIP family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes. JOURNAL OF CELLULAR BIOCHEMISTRY, 2018 (PubMed: 29923351) [IF=4.0]

Application: WB    Species: rat    Sample: cardiomyocytes

FIGURE 4| EndoA2 modulated the MAPK signaling pathway in response to ERS. A-F, Western blotting results show the expression levels of GRP78, p-PERK, p-eiF2α, ATF4, and CHOP after EndoA2 overexpression. Densitometric analyses show that EndoA2 overexpression decreased the expression levels of GRP78, p-PERK, p-eiF2α, ATF4, and CHOP, compared with those in the Ang II group. Transfection with AdlacZ had no significant effects (n = 5, *P < 0.05 vs control, #P < 0.05 vs Ang II).
2). POU4F3 Acts as a Tumor Suppressor in Lung Adenocarcinoma via the Endoplasmic Reticulum Stress Signaling Pathway. Journal of Cancer, 2022 (PubMed: 35069902) [IF=3.9]

Application: WB    Species: Human    Sample: LUAD cells

Figure 4 POU4F3 activated the ERS pathways in LUAD cells according to WB. A. The protein levels of PERK, p-PERK, eIF2α, p-eIF2α, ATF4, and CHOP in SPCA1 and A549 cells with or without POU4F3 overexpression, and with or without POU4F3 knockdown, were detected by WB. B. The protein levels of IRElα, p-IRElα, and XBP-1s in SPCA1 and A549 cells with or without POU4F3 overexpression, and with or without POU4F3 knockdown, were detected by WB.' ****', '***', and '**' represent P < 0.0001, 0.001, and 0.01 respectively.
3). Inhibition of the SIRT1 signaling pathway exacerbates endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in type 1 diabetic rats. Molecular Medicine Reports, 2020 (PubMed: 31974604) [IF=3.4]

Application: WB    Species: rat    Sample: renal

Figure 3. |DM enhances endoplasmic reticulum stress. Western blot analysis was performed after 24 h of reperfusion. (A) Representative blots and histograms showing (B) p‑PERK/PERK ratio; (C) p‑eIF2α/eIF2α ratio; (D) ATF4 expression; and (E) CHOP expression.
4). Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress-mediated apoptosis in vitro and in vivo. Oncology Letters, 2018 (PubMed: 30344724) [IF=2.9]

Application: WB    Species: human    Sample: GBC‑SD cells

Figure 1. |Rg3 activates ER stress in GBC‑SD cells. (A) GBC‑SD cells were treated with 1, 25, 50 and 100 µM Rg3 for 72 h, and proliferation was determined using a CCK‑8 kit. (B) Rg3 induced p‑eIF2α, ATF4 and Lcn2 expression in GBC‑SD cells. Relative protein expression level of (C) eIF2α, (D) p‑eIF2α, (E) ATF4 and (F) Lcn2 compared with the DMSO group. Relative protein expression was quantified by normalizing to the internal control β‑actin (n=3). *P<0.05, **P<0.01 vs. DMSO group. OD, optical density; ER, endoplasmic reticulum; eIF2α, eukaryotic translation‑initiation factor 2α; ATF4, activating transcription factor 4; p‑, phospho‑; DMSO, dimethylsulfoxide; Lcn2, lipocalin 2.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.