(4)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF5453, RRID:AB_2837937.
展开/折叠
5 HT 1A; 5 HT receptor 1A; 5 HT1A; 5 HT1A receptor; 5 hydroxytryptamine (serotonin) receptor 1A; 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled; 5 hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; 5-hydroxytryptamine receptor 1A; 5HT 1A; 5HT 1A receptor; 5HT receptor 1A; 5HT1a; 5HT1A_HUMAN; ADRB2RL1; ADRBRL1; G 21; G protein coupled receptor; G-21; Gpcr 18; Gpcr18; Guanine nucleotide binding regulatory protein-coupled receptor; Htr1a; PFMCD; Serotonin 1A receptor; Serotonin 5HT1A receptor; Serotonin receptor 1A;
抗原和靶标
Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells.
- P08908 5HT1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08908 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T196 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K324 | Acetylation | Uniprot | |
| K334 | Ubiquitination | Uniprot |
研究背景
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.
Cell membrane>Multi-pass membrane protein.
Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells.
Heterodimer; heterodimerizes with GPER1.
Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Sensory system > Taste transduction.
文献引用
Application: WB Species: Sample:
Application: IHC Species: Mouse Sample: cortex, hippocampus, and hypothalamus
限制条款
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