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产品:	Cyclin E2 抗体
货号:	AF5454
描述:	Rabbit polyclonal antibody to Cyclin E2
应用:	WB IHC IF/ICC
反应:	Human, Mouse
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量:	47 kDa; 47kD(Calculated).
蛋白号:	O96020
RRID:	AB_2837938

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)

克隆:

Polyclonal

特异性:

Cyclin E2 Antibody detects endogenous levels of total Cyclin E2.

RRID:

AB_2837938
引用格式: Affinity Biosciences Cat# AF5454, RRID:AB_2837938.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CCN E2; CCNE 2; CCNE2; CCNE2 protein; CYC E2; CYCE 2; CYCE2; CyclinE2; G1/S specific cyclin E2;

抗原和靶标

免疫原:

Uniprot:

O96020(CCNE2_HUMAN) >>Visit HPA database.

基因/基因ID:

CCNE2(9134)

表达:

O96020 CCNE2_HUMAN:

According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

描述:

The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1).

序列:

O96020 CCNE2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRRSSRLQAKQQPQPSQTESPQEAQIIQAKKRKTTQDVKKRREEVTKKHQYEIRNCWPPVLSGGISPCIIIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQMRSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDINKNMLQLIGITSLFIASKLEEIYAPKLQEFAYVTDGACSEEDILRMELIILKALKWELCPVTIISWLNLFLQVDALKDAPKVLLPQYSQETFIQIAQLLDLCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIPMEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O96020 作为底物

O96020

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
T19	Phosphorylation	Uniprot
S21	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
K49	Ubiquitination	Uniprot
S67	Phosphorylation	Uniprot
T74	Phosphorylation	Uniprot
S339	Phosphorylation	Uniprot
S383	Phosphorylation	Uniprot
T392	Phosphorylation	Uniprot
S396	Phosphorylation	Uniprot

研究背景

O96020_CCNE2_HUMAN

功能:

Essential for the control of the cell cycle at the late G1 and early S phase.

翻译修饰:

Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway.

细胞定位:

Nucleus.

组织特异性:

According to highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According tohighly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

亚基结构:

Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.

蛋白家族:

Belongs to the cyclin family. Cyclin E subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

文献引用

1). Thalidomide prevents antibody-mediated immune thrombocytopenia in mice. THROMBOSIS RESEARCH, 2019 (PubMed: 31670229) [IF=7.5]

Application: WB Species: mouse Sample: RAW264.7 cells

Fig. 6.| Expression of cyclin D1 and E2 in RAW264.7 cells. After treated with thalidomide, protein was extracted from RAW264.7 cells followed by analysis of the expression of cyclin D1 and E2 by western blot. Data were presented as mean ± SE (n = 6 for each group). Compared with 0, **P < 0.01;***P < 0.001.

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Cyclin E2 Antibody - #AF5454

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