VDAC1 Antibody - #AF5478

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产品: VDAC1 抗体
货号: AF5478
描述: Rabbit polyclonal antibody to VDAC1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 31 kDa; 31kD(Calculated).
蛋白号: P21796
RRID: AB_2837960

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
VDAC1 Antibody detects endogenous levels of total VDAC1.
RRID:
AB_2837960
引用格式: Affinity Biosciences Cat# AF5478, RRID:AB_2837960.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

N2441; OMP2; POR1; hVDAC1; MGC111064; Mitochondrial Porin; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; VDAC; VDAC-1; Vdac1; VDAC1_HUMAN; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; Voltage-dependent anion-selective channel protein 1; YNL055C; YNL2441C;

抗原和靶标

免疫原:
Uniprot:

P21796(VDAC1_HUMAN) >>Visit HPA database.

基因/基因ID:
VDAC1(7416)
表达:
P21796 VDAC1_HUMAN:

Heart, liver and skeletal muscle.

描述:
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV.
序列:
MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFDSSFSPNTGKKNAKIKTGYKREHINLGCDMDFDIAGPSIRGALVLGYEGWLAGYQMNFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P21796 作为底物

Site PTM Type Enzyme
A2 Acetylation
K12 Acetylation
K12 Ubiquitination
S13 Phosphorylation
T19 Phosphorylation
K20 Acetylation
K20 Ubiquitination
Y22 Phosphorylation
K28 Acetylation
K28 Ubiquitination
K34 Acetylation
K34 Ubiquitination
S43 Phosphorylation
S44 Phosphorylation
S46 Phosphorylation
K53 Ubiquitination
S57 Phosphorylation
K61 Acetylation
K61 Ubiquitination
Y62 Phosphorylation
T65 Phosphorylation
Y67 Phosphorylation
T98 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S104 Phosphorylation
T107 Phosphorylation
K109 Acetylation
K109 Sumoylation
K109 Ubiquitination
K110 Ubiquitination
K119 Acetylation
K161 Ubiquitination
R163 Methylation
T165 Phosphorylation
S167 Phosphorylation
Y173 Phosphorylation
K174 Acetylation
K174 Methylation
S193 Phosphorylation Q96PY6 (NEK1)
Y195 Phosphorylation
K197 Ubiquitination
T204 Phosphorylation
S215 Phosphorylation
K224 Acetylation
K224 Ubiquitination
Y225 Phosphorylation
C232 S-Nitrosylation
S234 Phosphorylation
K236 Acetylation
S240 Phosphorylation
S241 Phosphorylation
Y247 Phosphorylation
T250 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S260 Phosphorylation
K266 Acetylation
K266 Ubiquitination
K274 Acetylation
K274 Sumoylation
K274 Ubiquitination

研究背景

功能:

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

翻译修饰:

Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

细胞定位:

Mitochondrion outer membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Membrane raft>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart, liver and skeletal muscle.

亚基结构:

Interacts with hexokinases including HK1. The HK1-VDAC1 complex interacts with ATF2. Interacts with BCL2L1. Interacts with BAK1. Interacts with RTL10/BOP (via BH3 domain). Interacts with amyloid-beta and APP; induces VDAC1 dephosphorylation. Component of the mitochondrial permeability transition pore complex (mPTPC), at least composed of SPG7, VDAC1 and PPIF. Interacts with SPG7, NIPSNAP2 and SLC25A30.

(Microbial infection) Interacts with influenza A virus PB1-F2 protein.

蛋白家族:

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.

Belongs to the eukaryotic mitochondrial porin family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). The clinical antiprotozoal drug nitazoxanide and its metabolite tizoxanide extend Caenorhabditis elegans lifespan and healthspan. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39027239) [IF=14.5]
2). Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells. British Journal of Pharmacology, 2023 [IF=7.3]
3). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.8]
4). 3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2. Journal of Agricultural and Food Chemistry, 2023 (PubMed: 37750480) [IF=6.1]
5). α‑Phellandrene enhances the apoptosis of HT‑29 cells induced by 5‑fluorouracil by modulating the mitochondria‑dependent pathway. Oncology reports, 2024 (PubMed: 38456489) [IF=4.2]

Application: IF/ICC    Species: Human    Sample: HT-29 cells

Figure 3. Effect of α-PA combined with 5-FU treatment on VDAC-1 and HK-2 protein expression in HT-29 cells. HT-29 cells (1.0×105 cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. (A) Immunocytochemical staining was performed to determine the expression levels of HK-2 (green) and VDAC-1 (red) (Hoechst 33342 (blue) (×400 magnification). Quantitative analysis of the relative expression levels of (B) HK-2 and (C) VDAC-1 after treatment. Data are presented as the mean ± SD (n=3-5). *P
6). Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro. Biomed Research International, 2017 (PubMed: 28540295)

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