CDKN2A/p16INK4a Antibody - #AF5484

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(24)   (7)  
产品: CDKN2A/p16INK4a 抗体
货号: AF5484
描述: Rabbit polyclonal antibody to CDKN2A/p16INK4a
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Rabbit
分子量: 16 kDa; 17kD(Calculated).
蛋白号: P42771
RRID: AB_2837964

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(85%), Bovine(%), Horse(%), Rabbit(%)
克隆:
Polyclonal
特异性:
CDKN2A/p16INK4a Antibody detects endogenous levels of total CDKN2A/p16INK4a.
RRID:
AB_2837964
引用格式: Affinity Biosciences Cat# AF5484, RRID:AB_2837964.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cyclin-dependent kinase inhibitor 2A;Cyclin-dependent kinase 4 inhibitor A;CDK4I;Multiple tumor suppressor 1;MTS-1;p16-INK4a;p16-INK4;p16INK4A;CDKN2A;CDKN2;MTS1;

抗原和靶标

免疫原:
Uniprot:

P42771(CDN2A_HUMAN) >>Visit HPA database.

基因/基因ID:
CDKN2A(1029)
表达:
P42771 CDN2A_HUMAN:

Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

描述:
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
序列:
MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
85
Horse
85
Bovine
82
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

翻译修饰:

Phosphorylation seems to increase interaction with CDK4.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

亚基结构:

Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2.

蛋白家族:

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

文献引用

1). Senescent-like macrophages mediate angiogenesis for endplate sclerosis via IL-10 secretion in male mice. Nature communications, 2024 (PubMed: 38580630) [IF=16.6]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 6: Knockout of cdkn2a (p16) in Lyz2+ cells abrogates LSI-induced endplate sclerosis. a Representative immunofluorescent images of p16+ (red), F4/80+ (green) cells and DAPI (blue) staining of nuclei in mouse caudal endplates of L4/5 in Cdkn2aΔLyz2 or Cdkn2af/f mice at 8 weeks after LSI or sham surgery. Scale bars, 50 μm. b Representative immunofluorescent images of CD31 (green), Emcn (red) and DAPI (blue) staining of nuclei in the endplates of Cdkn2aΔLyz2 or Cdkn2af/f mice after LSI or sham surgery. Scale bars, 50 μm. c Permillage of CD31+Emcn+ area in the endplates of b. d Representative µCT images of the caudal endplates of L4/5 (coronal view) in Cdkn2aΔLyz2 or Cdkn2af/f mice at 8 weeks after LSI or sham surgery. Scale bars, 500 μm. e Quantitative analysis of the total porosity of d. f Representative images of safranin O and fast green staining of endplates in Cdkn2aΔLyz2 or Cdkn2af/f mice at 8 weeks after LSI or sham surgery. Scale bars, 50 μm. g Endplate scores of the endplates of f. h Representative immunohistochemical images of Osterix (Osx) in the endplates of Cdkn2aΔLyz2 or Cdkn2af/f mice at 8 weeks after LSI or sham surgery. Scale bars, 50 μm. i Quantitative analysis of the number of Osx+ cells in the endplates of h. j Representative images of immunofluorescent analysis of CGRP+ sensory nerves (red) and DAPI (blue) staining of nuclei in the endplates of Cdkn2aΔLyz2 or Cdkn2af/f mice after LSI or sham surgery. Scale bars, 50 μm. k Permillage of CGRP+ area in the endplates of l. n = 6 per group. Data are represented as means ± standard deviations, as determined by One-way ANOVA. Source data are provided as a Source Data file.
2). Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38502885) [IF=15.1]
3). Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis. Redox Biology, 2021 (PubMed: 33662874) [IF=10.7]

Application: WB    Species: mice    Sample: bone marrow mesenchymal stem (BMSCs)

Fig. 1. Effects of AGEs in different concentrations on the senescence of BMSCs. The BMSCs were treated with AGEs (50–200 μg/mL) or BSA for 24–72 h. (A) SA-β-gal assay for detection of BMSCs senescence. Scale bar: 100 μm. (B) Detection of H3K9me3 by immunofluorescence in BMSCs. Scale bar: 100 μm. (C) Detection of γ-H2AX by immunofluorescence in BMSCs. Scale bar: 100 μm. (D–I) Representative Western blotting assay and quantitation of the level of P16, P21, P53. **p < 0.01 versus BSA.
4). Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine, 2019 (PubMed: 30527623) [IF=9.7]
5). The brain-protective mechanism of fecal microbiota transplantation from young donor mice in the natural aging process via exosome, gut microbiota, and metabolomics analyses. Pharmacological research, 2024 (PubMed: 39053865) [IF=9.1]
6). VDR activation attenuates osteoblastic ferroptosis and senescence by stimulating the Nrf2/GPX4 pathway in age-related osteoporosis. Free Radical Biology and Medicine, 2022 (PubMed: 36402439) [IF=7.1]
7). Dihydromyricetin regulates the miR-155-5p/SIRT1/VDAC1 pathway to promote liver regeneration and improve alcohol-induced liver injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 39986231) [IF=6.7]
8). Oxygen–Glucose Deprivation Promoted Fibroblast Senescence and Collagen Expression via IL11. International Journal of Molecular Sciences, 2022 (PubMed: 36292942) [IF=5.6]
9). Research on the anti-aging mechanisms of Panax ginseng extract in mice: a gut microbiome and metabolomics approach. Frontiers in pharmacology, 2024 (PubMed: 38966558) [IF=5.6]
10). Mechanistic study of the regulation of mitochondrial function by the GPNMB/Nrf2/NF-κB signaling pathway mediated by Quzhi Tang to alleviate chondrocyte senescence. Journal of ethnopharmacology, 2024 (PubMed: 39617085) [IF=5.4]
加载更多

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.