PGP9.5 Antibody - #AF5490

Fig. 9. The antiviral effect of LanC in vivo. (A) Mice were inoculated with HSV-1 (1 × 106 plaque-forming units) on the shin of the right hind paw. LanC (0.6 mg/kg and 1.2 mg/kg) or ACV (10 mg/kg) was administered intraperitoneally daily after HSV-1 inoculation. The mechanical allodynia was detected on day 7 after HSV-1 infection. The data presented are means and SD (n = 6). (B) Confocal analysis of intra-epidermal nerve fibers (PGP9.5; green) from the plantar of the right hind paws. Merge images with the nuclear marker DAPI (blue). (C) Effect of LanC on PGP9.5 protein expression in the plantar of the right hind paws of mice after HSV-1 inoculation. (D) Results from three independent experiments were quantitated and presented as means and SD. (E) The expression of virus IE gene ICP0, early gene UL23, glycoprotein G-encoding gene US4 and latency-associated gene LAT in DRG was detected by RT-qPCR. LanC, Lanatoside C; ACV, acyclovir; PGP9.5, Protein Gene Product 9.5; HSV-1, herpes simplex virus type 1; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. #### p < 0.0001 vs. Sham group; * p < 0.05, ** p < 0.01, **** p < 0.001, **** p < 0.0001 vs. HSV-1

Fig. 9. The antiviral effect of LanC in vivo. (A) Mice were inoculated with HSV-1 (1 × 106 plaque-forming units) on the shin of the right hind paw. LanC (0.6 mg/kg and 1.2 mg/kg) or ACV (10 mg/kg) was administered intraperitoneally daily after HSV-1 inoculation. The mechanical allodynia was detected on day 7 after HSV-1 infection. The data presented are means and SD (n = 6). (B) Confocal analysis of intra-epidermal nerve fibers (PGP9.5; green) from the plantar of the right hind paws. Merge images with the nuclear marker DAPI (blue). (C) Effect of LanC on PGP9.5 protein expression in the plantar of the right hind paws of mice after HSV-1 inoculation. (D) Results from three independent experiments were quantitated and presented as means and SD. (E) The expression of virus IE gene ICP0, early gene UL23, glycoprotein G-encoding gene US4 and latency-associated gene LAT in DRG was detected by RT-qPCR. LanC, Lanatoside C; ACV, acyclovir; PGP9.5, Protein Gene Product 9.5; HSV-1, herpes simplex virus type 1; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. #### p < 0.0001 vs. Sham group; * p < 0.05, ** p < 0.01, **** p < 0.001, **** p < 0.0001 vs. HSV-1

Figure 4 (A) Normal group, (B) Model group, (C) Treatment group: Rabbit SO tissue, nerve fiber PGP9.5 in the inner muscular layer detected by laser confocal microscopy, red light is PGP9.5, blue light is DAPI. (D) Normal group, (E) Model group, (F) SGD treatment group: α-actin labeled rabbit SO tissues were examined by laser confocal microscopy (400×, 20 um), with α-actin in red light and DAPI in blue light. (G) fluorescence intensity of PGP9.5 (IOD) in the SO tissue of rabbits in each group; (H) The fluorescence intensity (IOD) of α-actin labeled SMC in the SO tissue of rabbits in each group. Arrows indicate positive expression.*** indicates the comparison between the model group and the control group, P<0.05, ##indicates the comparison between the model group and the treatment group, P<0.05. In α-action, ** indicates the comparison between the model group and the control group, P<0.05, # indicates the comparison between the model group and the treatment group, P<0.05.