产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6003, RRID:AB_2837980.
展开/折叠
Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB_HUMAN; PHB1; Prohibitin;
抗原和靶标
- P35232 PHB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35232 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K4 | Acetylation | Uniprot | |
| K4 | Methylation | Uniprot | |
| K4 | Ubiquitination | Uniprot | |
| K11 | Methylation | Uniprot | |
| R41 | Methylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| R93 | Methylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y114 | Phosphorylation | P06213 (INSR) | Uniprot |
| S121 | O-Glycosylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| K128 | Acetylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K202 | Acetylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K207 | Acetylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T258 | O-Glycosylation | Uniprot | |
| T258 | Phosphorylation | P31749 (AKT1) | Uniprot |
| Y259 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot |
研究背景
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Mitochondrion inner membrane. Nucleus.
Widely expressed in different tissues.
Interacts with PHB2. Interacts with STOML2.
Belongs to the prohibitin family.
限制条款
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