AFP Antibody - #DF6007

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产品: AFP 抗体
货号: DF6007
描述: Rabbit polyclonal antibody to AFP
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 65kDa; 69kD(Calculated).
蛋白号: P02771
RRID: AB_2837984

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:10-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
AFP Antibody detects endogenous levels of total AFP.
RRID:
AB_2837984
引用格式: Affinity Biosciences Cat# DF6007, RRID:AB_2837984.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Afp; AFPD; Alpha fetoglobulin; Alpha fetoprotein; Alpha fetoprotein precursor; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; alpha-fetoprotein, Hereditary persistence of, included; FETA; FETA_HUMAN; Hereditary persistence of alpha fetoprotein; HPAFP;

抗原和靶标

免疫原:
Uniprot:

P02771(FETA_HUMAN) >>Visit HPA database.

基因/基因ID:
AFP(174)
表达:
P02771 FETA_HUMAN:

Plasma. Synthesized by the fetal liver and yolk sac.

描述:
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).
序列:
MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
88
Sheep
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02771 作为底物

Site PTM Type Enzyme
S111 Phosphorylation
S115 Phosphorylation
S117 Phosphorylation
T248 Phosphorylation
S344 Phosphorylation
S368 Phosphorylation
K413 Acetylation
S415 Phosphorylation
S444 Phosphorylation
S445 Phosphorylation

研究背景

功能:

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

翻译修饰:

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.

Sulfated.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Plasma. Synthesized by the fetal liver and yolk sac.

亚基结构:

Dimeric and trimeric forms have been found in addition to the monomeric form.

蛋白家族:

Belongs to the ALB/AFP/VDB family.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

文献引用

1). Disordered farnesoid X receptor signaling is associated with liver carcinogenesis in Abcb11-deficient mice. The Journal of pathology, 2021 (PubMed: 34410012) [IF=7.3]
2). Piezo1 impairs hepatocellular tumor growth via deregulation of the MAPK-mediated YAP signaling pathway. Cell Calcium, 2021 (PubMed: 33610907) [IF=4.0]

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