ATF6 Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	ATF6 抗体
货号:	DF6009
描述:	Rabbit polyclonal antibody to ATF6
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
分子量:	50~75kD(cleaved),90~100kD(full); 75kD(Calculated).
蛋白号:	P18850
RRID:	AB_2833019

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

ATF6 Antibody detects endogenous levels of total ATF6.

RRID:

AB_2833019
引用格式: Affinity Biosciences Cat# DF6009, RRID:AB_2833019.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Activating transcription factor 6 alpha; Activating transcription factor 6; ATF 6; ATF6 alpha; ATF6; ATF6-alpha; ATF6A; ATF6A_HUMAN; cAMP dependent transcription factor ATF 6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Cyclic AMP dependent transcription factor ATF 6 alpha; DKFZp686P2194; ESTM49; FLJ21663; Processed cyclic AMP dependent transcription factor ATF 6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha;

抗原和靶标

免疫原:

Uniprot:

P18850(ATF6A_HUMAN) >>Visit HPA database.

基因/基因ID:

ATF6(22926)

表达:

P18850 ATF6A_HUMAN:

Ubiquitous.

描述:

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

序列:

P18850 ATF6A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQLEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSSPRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTENGLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIISLQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSPVNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLEQDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQGSNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHLLLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPPYLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Bovine

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18850 作为底物

P18850

Site	PTM Type	Enzyme	Source
S16	Phosphorylation		Uniprot
K87	Sumoylation		Uniprot
K87	Ubiquitination		Uniprot
S94	Phosphorylation		Uniprot
Y100	Phosphorylation		Uniprot
S104	Phosphorylation		Uniprot
K152	Sumoylation		Uniprot
T166	Phosphorylation	Q16539 (MAPK14)	Uniprot
K176	Acetylation		Uniprot
K176	Sumoylation		Uniprot
K176	Ubiquitination		Uniprot
K182	Sumoylation		Uniprot
K191	Sumoylation		Uniprot
K201	Ubiquitination		Uniprot
K216	Ubiquitination		Uniprot
K290	Sumoylation		Uniprot
K290	Ubiquitination		Uniprot
T296	Phosphorylation		Uniprot
Y330	Phosphorylation		Uniprot
K339	Ubiquitination		Uniprot
K349	Ubiquitination		Uniprot
S373	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
K424	Ubiquitination		Uniprot
K436	Ubiquitination		Uniprot
N472	N-Glycosylation		Uniprot
K506	Ubiquitination		Uniprot
T565	Phosphorylation		Uniprot
N584	N-Glycosylation		Uniprot
K629	Ubiquitination		Uniprot
S632	Phosphorylation		Uniprot
N643	N-Glycosylation		Uniprot

研究背景

P18850_ATF6A_HUMAN

功能:

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

翻译修饰:

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.

N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).

Phosphorylated in vitro by MAPK14/P38MAPK.

细胞定位:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Nucleus.
Note: Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.

组织特异性:

Ubiquitous.

亚基结构:

Homodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). Interacts also with the transcription factors GTF2I, YY1 and SRF. Interacts (via lumenal domain) with THBS1 (By similarity). Interacts with THBS4 (via EGF-like 3; calcium-binding domain) which facilitates its processing, activation and nuclear translocation. Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner.

蛋白家族:

The basic domain functions as a nuclear localization signal.

The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.

Belongs to the bZIP family. ATF subfamily.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

文献引用

1). Branched chain amino acids exacerbate myocardial ischemia/reperfusion vulnerability via enhancing GCN2/ATF6/PPAR-α pathway-dependent fatty acid oxidation. Theranostics, 2020 (PubMed: 32373236) [IF=12.4]

Application: WB Species: mouse Sample: Heart

Figure 6. BCAA increase PPAR-α expression in a GCN2/ATF6 pathway-dependent manner. (A) Expression of p-GCN2, GCN2 and ATF6 in the presence of increasing concentrations of BCAA (0, 0.429 mM, 0.858 mM, 1.716 mM, 3.432 mM) by western blotting (n=6). (B) Expression of p-GCN2, GCN2 and ATF6 in the presence of increasing concentrations of BCKA (0, 0.429 mM, 0.858 mM, 1.716 mM, 3.432 mM) by western blotting (n=6). BCKA mixture is composed of αKIC, αKIV and αKMV (weight ratio, αKIC: αKIV: αKMV= 2:1:1). (C) NRVMs were treated with control siRNA and ATF6 siRNA. 48 h after transfection, expression of ATF6 was determined by western blotting (n=4). (D-E) ATF6 siRNA transferred NRVMs were treated with or without BCAA (3.432 mM) (n=6). (D) PPAR-α expression was determined by western blotting. (E) Expression of Acaa2, Acadm, Cd36 and Cpt1b by real-time PCR. (F-G) ATF6 siRNA transferred NRVMs were treated with or without BCKA (3.432 mM) (n=6). (F) PPAR-α expression was determined by western blotting. (G) Expression of Acaa2, Acadm, Cd36 and Cpt1b by real-time PCR. (C) Data were analyzed by Student’s t test. (A-B) and (D-G) Data were analyzed by one-way ANOVA, followed by a Bonferroni post-hoc test. * P<0.05, ** P<0.01. All values are presented as mean ± SEM.

2). Sleep Deprivation Induces Gut Damage via Ferroptosis. Journal of pineal research, 2024 (PubMed: 38975671) [IF=10.3]

3). PPM1H is down-regulated by ATF6 and dephosphorylates p-RPS6KB1 to inhibit progression of hepatocellular carcinoma. Molecular therapy. Nucleic acids, 2023 (PubMed: 37456776) [IF=8.8]

Application: WB Species: Human Sample: Hep-G2 cells

Figure 4. PPM1H directly dephosphorylated p-RPS6KB1 A–C) 24 h after transfection, cells were cultured in basal medium without serum overnight; insulin (10 ng/μL) was added 2 h before harvest to activate phosphorylation of RPS6KB1. (A) FLAG-PPM1H was transfected into Hep-G2 cells with or without insulin treatment, and coIP was performed to examine the level of RPS6KB1 combined with PPM1H. (B) CoIP analysis of PPM1H and RPS6KB1 in FLAG-RPS6KB1-transfected Hep-G2 cells with or without insulin treatment. (C) Western blot analysis of the indicated protein levels in PPM1H-overexpressing Hep-G2 cells with or without insulin treatment. (D) In vitro phosphatase assay to determine the interaction between PPM1H and p-RPS6KB1. (E and F) Western blot analysis of the indicated protein levels in vector, ATF6, sh-NC, and sh-ATF6 transfected Hep-G2 cells (E) and in liver tissues of Atf6fl/fl or Atf6Δhep mice. (G) The RPS6KB1 inhibitor PF-4708671 was added to cells at a concentration of 10 μM, which effectively blocked phosphorylation of p-RPS6KB1. Transwell assays revealed that the migration and invasion of PF-4708671-treated Hep-G2 and Huh-7 cells showed little difference between vector and PPM1H. Magnification, ×100. (H) Quantification of (G). Data represent the mean ± SD of three independent experiments. ns, not significant.

4). Zonisamide, an antiepileptic drug, alleviates diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress. Acta Pharmacologica Sinica, 2020 (PubMed: 32647341) [IF=8.2]

Application: WB Species: mice Sample: NRCMs

Fig. 6 Zonisamide alleviates HG-induced cardiac hypertrophy and apoptosis in cultured primary neonatal rat cardiomyocytes (NRCMs) via suppression of activated ER stress. NRCMs were pretreated with 5 mM 4-PBA (an inhibitor of ERS) or 10 ng/mL tunicamycin (Tm, an ERS inducer) for 2 h and then exposed to glucose (33 mM) in the presence or absence of ZNS (3 μM) for 24 h. a–b Representative and quantitative images showing the protein expression of ERS markers, including GRP78, XBP-1s, ATF6, p-PERK, PERK, ATF4, CHOP, and Hrd1. c Immunofluorescence staining of cardiomyocytes with phalloidin (red) and cell nuclei with DAPI (blue), Scale bar = 50 μm. d Quantitative analysis of cell surface area by ImageJ software. e–f Representative Western blotting and analysis of Bax and Bcl-2 expression. g–h Representative and quantitative images of GRP78, ATF6, p-PERK, PERK, ATF4, and CHOP expression. All values are the fold changes normalized to their control group. The results are presented as the means ± SEM (n = 6). *P < 0.05, **P < 0.01 vs. Con; #P < 0.05, ##P < 0.01 vs. HG; $P < 0.05, $$P < 0.01 vs. HG + ZNS.

5). Patulin induces ROS-dependent cardiac cell toxicity by inducing DNA damage and activating endoplasmic reticulum stress apoptotic pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38061079) [IF=6.8]

6). Oleic acid protects saturated fatty acid mediated lipotoxicity in hepatocytes and rat of non-alcoholic steatohepatitis. LIFE SCIENCES, 2018 (PubMed: 29709653) [IF=6.1]

7). Network-based identification and mechanism exploration of active ingredients against Alzheimer's disease via targeting endoplasmic reticulum stress from traditional chinese medicine. Computational and structural biotechnology journal, 2024 (PubMed: 38261917) [IF=6.0]

8). The potential immunotoxicity of fine particulate matter based on SD rat spleen. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2019 (PubMed: 31218585) [IF=5.8]

Application: WB Species: rat Sample: spleen

Fig. 3 | PM2.5 induced ERS in spleen of SD rats. a, b The levels of spleen GRP78 and ATF6 mRNA were measured using qRT-PCR by summer and winter PM2.5 treatment in SD rat. c, dThe levels of spleen GRP78 and ATF6 protein were measured using western blotting in summer PM2.5 treatment in SD rats and quantification of analysis

9). Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling. Frontiers in Pharmacology, 2023 (PubMed: 37021049) [IF=5.6]

10). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB Species: rat Sample: liver

FIGURE 4 | PA treatment attenuated HFD-induced ER stress in rats. (A) Representative immunoreactive bands of GRP78, PERK, p-PERK, IRE1α, p-IRE1α, and ATF6

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ATF6 Antibody - #DF6009

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