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| 产品: | BMI1 抗体 |
| 货号: | DF6017 |
| 描述: | Rabbit polyclonal antibody to BMI1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Chicken |
| 分子量: | 37kDa; 37kD(Calculated). |
| 蛋白号: | P35226 |
| RRID: | AB_2837992 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6017, RRID:AB_2837992.
展开/折叠
B lymphoma Mo MLV insertion region (mouse); B lymphoma Mo MLV insertion region 1 homolog; Bmi 1; BMI1; BMI1 polycomb ring finger oncogene; BMI1_HUMAN; Flvi 2/bmi 1; FLVI2/BMI1; MGC12685; Murine leukemia viral (bmi 1) oncogene homolog; Oncogene BMI 1; PCGF 4; PCGF4; Polycomb complex protein BMI 1; Polycomb complex protein BMI-1; Polycomb group protein Bmi1; Polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RNF 51; RNF51;
抗原和靶标
- P35226 BMI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35226 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K73 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K88 | Sumoylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| R114 | Methylation | Uniprot | |
| K151 | Acetylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| Y204 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | P31749 (AKT1) | Uniprot |
研究背景
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Nucleus. Cytoplasm.
Component of a PRC1-like complex. Identified in a PRC1-like HPRC-H complex with CBX2, CBX4, CBX8, PHC1, PHC2, PHC3 RING1 and RNF2. Interacts with RNF2/RING2. Interacts with RING1 (By similarity). Part of a complex that contains RNF2, UB2D3 and BMI1, where RNF2 and BMI1 form a tight heterodimer, and UB2D3 interacts only with RNF2. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. Interacts with CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Interacts with PHC2.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
文献引用
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